The paper is organized as follows. Section “TheNETMEmodel” introducesNETMEsystem together with its components. Section “The annotation tool” provides the technical details of the back-end and the front-end ofNETME. Section “Experimental analysis” reports two different case studies that allow ...
7. Role of SIRT3 in HF and NETosis SIRT3 is a major mitochondrial deacetylase and an important factor in maintaining cardiac mitochondrial bioenergy. Multiple studies have revealed that SIRT3 deficiency or decreased expression can induce the excessive acetylation of pyruvate dehydrogenase, ATP synthase...
Using this approach, we found that pretreatment of neutrophils with shear stress increased NETosis induced by LPS and ATP, as evidenced by the increased NET area and degree of H3 citrullination, but did not affect PMA -induced NETosis (Fig. 1e, f). Neutrophil activation and NETosis are ...
Diagram showing the effect of the elimination from capacitation network of the most linked nodes. The elimination from capacitation network of the most linked nodes ([Ca2+]i and ATP-ADP) caused the collapse of network structure. Full size image Figure 5 Diagram showing the effect of the elimi...
Previous experimental evidence indicates that some cancer cells have an alternative glycolysis pathway with net zero ATP production, implying that upregula... Vazquez,Markert,EK,... - 《Plos One》 被引量: 108发表: 2011年 Thermoeconomic analysis of a novel zero-CO2-emission high-efficiency power...
The effect of inhibition of the mitochondrial ATPase with oligomycin on the rate of ATP depletion and anaerobic glycolysis was studied in the totally ischemic dog heart. An oxygenated, buffered crystalloidal solution containing 10 渭m oligomycin and 12 mm glucose was delivered at 100 mmHg pressure ...
(to minimize AE2a anion exchange activity), the addition of excess ATP (10 mM) resulted in marked acidification of the Golgi lumen (Fig.3A top panel) before it plateaued at pH 5.0–5.5 both in AE2a overexpressing and mock-transfected control cells, respectively. The calculated initial ...
(A)fructose-2,6-bisphosphate,(B)ATP,(C)ADP,(D)AMP,(E)CO2. Ifradioactiveinorganicphosphate(32~i) isaddedtoacellextractundergoingglycolysis,the32~icould bedirectlyincorporatedintoaglycolyticintermediatebywhichstep?(A) glucose+glucose-6-phosphate,(B)fructose-6-phosphate+fructose-1,6-bisphosphate,(C...
The voltage across the mitochondrial membrane drives ATP synthesis. High glucose levels enhance glycolysis, increasing electron donation to the ETC. This elevation in electron flux raises the membrane potential, leading to increased electron donation to oxygen, generating reactive oxygen species (ROS), ...
(AICAR)-treated bovine PMN. This finding correlated with upstream responses on the level of CAMKK activation. Moreover, these reactions were accompanied by an augmented autophagic activity, as represented by enhanced expression of ULK-1 but not of Beclin-1. Referring to neutrophil effector ...