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arebothavailable.Smallfeetonthebottomcornersofeachplateeliminatescrapingofthe microscopelens. 2.Markingsofwellcoordinatesavailablefor12,24,48,96and384wellplates 3.TCtreated:VacuumPlasmatissueculturetreatment,betterhydrophilicityforadherent cellculture.Non-treatedgrowthsurfaceismorehydrophobicthantissueculturesurface ...
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To assess the antimicrobial efficacy of MG extracts (Fig.3d), we quantified the increase over time of the OD in a 96-well plate box, with the outer row filled with 70 μL of PBS. In total we did 3 replicates per sample with a total of 70 μL per sample. Negative control: 70...
Toxicity study was first performed with addition of EBN extracts to SH-SY5Y cells to determine the concentration-wide effect as well as MNTDs of the extracts on neuronal culture. MNTD is the maximal dose just below the threshold for cell toxicity that demonstrates no cytotoxic effect. Half of ...
Macrophages were then detached from the plate by scraping. For infections, macrophages were seeded at 2 × 105 cells per well and infected at an MOI of 5 CFU/cell with bacteria in DMEM. Infection was carried out at 37°C for 30 min. After infection, cells were washed thoroughly with ...
Toxicity study was first performed with addition of EBN extracts to SH-SY5Y cells to determine the concentration-wide effect as well as MNTDs of the extracts on neuronal culture. MNTD is the maximal dose just below the threshold for cell toxicity that demonstrates no cytotoxic effect. Half of ...
C. stercoreus may be commonly found on dung, but urbanization and modern landscaping techniques have created a vast amount of new habitat for it as well other species of Nidulariaceae. Mulched woodpiles in urban and rural environments seem to be ideal substrates for some species of Nidulari...