p40 nuclear and DSG-3 cytoplasmic/membranous immunoreactivity in greater than or equal to 10% of thymic epithelial cells was interpreted as positive, and DSG-3 distribution pattern was classified as organotypic and nonorganotypic. All nonneoplastic thymic tissues, 100% of thymic squamous carcinomas,...
1a–c). These long spindle-shaped cells were positive for CD45 and Collagen-I. We next evaluated the characteristics of these cells using immunofluorescence. As shown in Fig. 1d, the cells produced collagen and fibronectin, and were positive for hematopoietic markers such as CD45, CD11b, ...
Interestingly, we found that decreased levels of Tregs appeared concomitantly with a decrease in CD4+CD25+ T helper (Th) cells, Scientific Reports | 7:40373 | DOI: 10.1038/srep40373 2 www.nature.com/scientificreports/ Figure 1. Tissue distribution of E171 particles in the rat intestine ...
Figure 3 Western blot analysis Cells were lysed in lysis buffer (50 mM Tris-HCl, pH 7.4, 150 mM NaCl, 1% Nonidet P40, 1 mM Na3VO4, 1 mM phenylmeth- ylsulphonyl fluoride, 1 µg/ml leupeptin, and 10 µg/ml apro- tinin). Cell lysates (50 µg of protein) were ...
In all 5 cases, the proliferative ducts were completely surrounded by a layer of myoepithelial cells that were positive for p63 or p40, smooth muscle actin, and calponin. Using combined HE, calponin immunofluorescence, and RET FISH imaging, the positive signals were unmistakably identified in both...