Neonatal rat cardiac fibroblasts express three types of voltage-gated Kϩ channels: regulation of a transient outward current by protein kinase C. Am J Physiol Heart Circ Physiol 294: H1010 -H1017, 2008.Neonatal rat cardiac fibro-blasts express three types of voltage-gated K+chan-nels:...
Carter RL, Grisanti LA, Yu JE, et al. Dynamic mass redistribution analysis of endogenous beta‐adrenergic receptor signaling in neona- tal rat cardiac fibroblasts. Pharmacology research & perspectives. 2014;2:1.Carter, RL, Grisanti, LA, Yu, JE, Repas, AA, Woodall, M, Ibetti, J, Koch, ...
Angiotensin II has been reported to be a hormonal stimulus of cardiac growth, a response that may involve myocyte hypertrophy as well as growth of nonmyocytes. This study was designed to determine whether neonatal rat cardiac fibroblasts have an angioten
Beta-adrenergic receptor-mediated DNA synthesis in neonatal rat cardiac fibroblasts proceeds via a phosphatidylinositol 3-kinase dependent pathway re- fractory to the antiproliferative action of cyclic AMP. J Cell Physiol. 2003;195:322-330.Colombo F, Gosselin H, El-Helou V, Calderone A: β-...
AIM:To investigate the effects of erythropoietin (EPO) on the proliferation of rat cardiac fibroblasts induced by angiotensin Ⅱ(Ang Ⅱ) and to identify the roles of phosphatidylinositol-3-kinase/Akt (PI3-K/Akt) signaling pathway and nitric oxide synthase (NOS) in this process. METHODS: Neonata...
Cardiac catheterization could also be performed on 30-day-old rats to assess the RV pressure. In summary, the PVS rat model introduced in our study provides a sound platform for future studies of PVS and its effects.Availability of data and materials All of the data in the current study is...
Aim:To determine whether all-trans retinoic acid(atRA)acts to modulate angio- tensin Ⅱ(Ang Ⅱ)-induced cardiac fibroblast cell growth and collagen secretion. Methods:Cultured neonatal rat cardiac fibroblasts(CF)were used in the experiment. A 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazoliu...
and down-regulated the activity of Brg1 and the expression of TGF-β1 induced by Ang II in cultured neonatal rat cardiomyocytes and cardiac fibroblasts, ... HP Qi,Y Wang,QH Zhang,... - 《Cellular Physiology & Biochemistry》 被引量: 45发表: 2015年 PPARγ-Activating Angiotensin Type-1 Rece...
Cardiomyocytes were enriched by preplating for 90 min to eliminate fibroblasts. More than 90% of the cells were identified as cardiac myocytes by immu- nocytochemistry with anti-sarcomeric β-actin (Sigma). The cells were then cultured in DMEM with 10% FBS, 100 U/mL penicillin, and 100 mg...
Preparation of neonatal CMs and adult cardiac fibroblasts Hearts were extracted from neonatal mice (P7) and immediately transferred into the dish containing 1× PBS on ice and washed twice. Subsequently, heart were transferred into the isolation medium (20 mM BDM, 0.0125% trypsin, in HBSS) and...