The neoglycolipid ( NGL ) technology is the basis of a state‐of‐the‐art oligosaccharide microarray system, which we offer for screening analyses to the broad scientific community. We review here the sequential development of the technology and its power in pinpointing and isolating naturally ...
Another such comprehensive glycan microarray is based on neoglycolipid (NGL) technology, where synthetic and natural glycans are conjugated to a hydrophobic lipid via microscale reductive amination or oxime ligation and purified to construct a NGL library, which is printed directly on and absorbed to ...
Preparation of NGL Probes The glucan oligosaccharides and the glucosylated high-mannose N-glycans prepared above were converted into oxime-linked NGLs (AO-NGLs) for recognition studies in microarrays. The step by step procedures for the preparation, purification, and quantitation of the AO-NGLs are...
The neoglycolipid (NGL) technology is the basis of a state-of-the-art oligosaccharide microarray system. The NGL-based microarray system in the Glycosciences Laboratory Imperial College London (http://www3.imperial.ac.uk/glycosciences) is one of the two leading platforms for glycan microarrays,...
The neoglycolipid (NGL) technology is the basis of a state-of-the-art oligosaccharide microarray system. The NGL-based microarray system in the Glycosciences Laboratory Imperial College London (http://www3.imperial.ac.uk/glycosciences) is one of the two leading platforms for glycan microarrays,...
We have developed a unique carbohydrate microarray system based on the neoglycolipid (NGL) technology, a well-validated microscale approach for generating lipid-tagged oligosaccharide probes for use in carbohydrate recognition studies. This chapter provides an overview of the principles and key features ...