More recently, there is a trend to differentiate corn not only on the basis of alterations in carbohydrate quality but also on the basis of its protein, oil, and kernel hardness characteristics. Several companies market corn with increased lysine (Crow's Hybrid Corn Company, Milford, Ill.), ...
A size exclusion chromatography (SEC) system comprised of a Model Alliance® 2690 from Waters Corporation (Milford, Mass. USA), with a Waters® 410 refractive index detector (DRI) and Viscotek Corp. (Houston, Tex., USA) Model T6OATM dual detector module incorporating static right angle ligh...
(Waters Corporation, Milford, Mass.) and combined with dry air fed from a calibrated mass flow controller (200 sccm maximum) to evaporate the water within a coil of 1.6 mm diameter stainless steel tubing inside the oven. The resulting humidified air can be fed into the inlet of the ...
Samples were injected onto a Shodex SH-1011 column (8 mm×300 mm, purchased from Waters, Milford, Mass.) equipped with a Shodex SH-1011P precolumn (6 mm×50 mm), temperature controlled at 50° C., using 0.01 N H2SO4 as mobile phase at a flow rate of 0.5 mL/min. When ...
Aliquots (250 μl) were removed from each well, transferred to clean 300 μl chromatography vials, and capped. The vials were heated in a 55° C. oven for 30 minutes. One hundred μl of each sample was injected into a Waters 2690 Separations Module (Milford, Mass.) having a series of...
Cell culture samples were taken at timed intervals and analyzed for EtOH and xylose using either a Waters HPLC system (Alliance system, Waters Corp., Milford, Mass.) or an Agilent 1100 Series LC; conditions=0.6 mL/min of 0.01 N H2SO4, injection volume=10 μL, autosampler temperature=10°...
Riston®: photopolymer resist equipment and stripping agents. Shodex® is a tradename of Water's Associates, Milford, Mass. for polystyrene-based chromatographic separation materials. J&S 732 is a tradename of J&S Laboratories, Los Angeles, Calif. for alkaline aqueous glycol ether/alkanolamine...
To analyze for 2,3-dihydroxyisovalerate, the sample was diluted 10× with water, and 8.0 μl was injected into a Waters Acquity HPLC equipped with Waters SQD mass spectrometer (Waters Corporation, Milford, Mass.). The chromatography conditions were: flow rate (0.5 ml/min), on a Waters ...
Molecular weight determination. A size exclusion chromatography system comprised of a Model Alliance 2690™ from Waters Corporation (Milford, Mass.), with a Waters 410™ refractive index detector (DRI) and Viscotek Corporation (Houston, Tex.) Model T-60A™ dual detector module incorporating stat...
The present invention provides an improved method for the biological production of 1,3-propanediol from a fermentable carbon source in a single microorganism. In one aspect of the p