NLRP3炎症体激活蛋白NLRP3、ASC、Caspase-1和效应蛋白IL-18和IL-1β的WB结果见图3E-I。模型组中NLRP3、ASC、Caspase-1、IL-18和IL-1β蛋白的水平明显增加,而QSG的干预则减少了上述蛋白的表达。 Figure 4 QSG inhibited LPS combined with ATP-induced NLRP3 inflammasome activation in RAW264.7 macrophages. ...
炎症小体(inflammasome)是能够激活炎性caspase蛋白激酶的胞内蛋白复合体,它对于机体炎症的发生与控制具有重要的调节作用。至 今为止,已经有4类炎症小体被发现:NLRP1、NLRP3、NLRC4以及AIM2。NLRP3发生突变能够引发许多炎性疾病,包括冷吡啉相关周期性综 合征(CAPS)。目前已经知道钾离子的外流对于NLRP3的外流具有重...
2016年2月24日讯 炎症小体(inflammasome)是能够激活炎性caspase蛋白激酶的胞内蛋白复合体,它对于机体炎症的发生与控制具有重要的调节作用。至今为止,已经有4类炎症小体被发现:NLRP1、NLRP3、NLRC4以及AIM2。 NLRP3发生突变能够引发许多炎性疾病,包括冷吡啉相关周期性综合征(CAPS)。目前已经知道钾离子的外流对于NLR...
之前的研究表明细胞内钾离子外流促进NLRP3和NEK7的结合,导致NLRP3炎症小体的激活【2】。但其分子机制未知。 2025年1月3日,密西根大学Gabriel Nunez和韦恩州立大学Yuan He研究组在Science Immunology杂志上发文NEK7 phosphorylation amplifies NLRP3 inflamm...
As an NLRP3-interacting partner, NEK7 has emerged as a critical mediator for NLRP3 inflammasome activation. In contrast to NEK7, NEK6, the closely related member of the NEK family, does not support NLRP3 inflammasome activation. Here, we show that the NEK7 catalytic domain, which shares a...
3. Bauer C, Duewell P, Mayer C, Lehr HA, Fitzgerald KA, Dauer M, et al. Colitis induced in mice with dextran sulfate sodium (DSS) is mediated by the NLRP3 inflammasome. Gut 2010;59:1192-9. 4. Kim JS, Kim HK, Lee J, Jang S, Cho E, Mun SJ, et al. Inhibition of CD82 im...
2016年2月24日 讯 /艾科试剂/ --炎症小体(inflammasome)是能够激活炎性caspase蛋白激酶的胞内蛋白复合体,它对于机体炎症的发生与控制具有重要的调节作用。至今为止,已经有4类炎症小体被发现:NLRP1、NLRP3、NLRC4以及AIM2。NLRP3发生突变能够引发许多炎性疾病,包括冷吡啉相关周期性综合征(CAPS)。目前已经知道钾离...
The NLRP3 inflammasome can be activated by stimuli that include nigericin, uric acid crystals, amyloid-β fibrils and extracellular ATP. The mitotic kinase NEK7 licenses the assembly and activation of the NLRP3 inflammasome in interphase. Here we report a cryo-electron microscopy structure of inacti...
Structural mechanism for NEK7-licensed activation of NLRP3 inflammasomewww.nature.com/articles/s41586-019-1295-z 模型建立与展示 使用Phyre2服务器24生成了NLRP3初始同源模型。在Chimera 50和Phenix 51的帮助下,在初始模型的不同部分进行了手动调整,刚体拟合,柔性拟合和基于段的实际空间优化,以适应Coot 49中...
The NLRP3 inflammasome plays a key role in responding to pathogens, and endogenous damage and mitochondria are intensively involved in inflammasome activation. The NLRP3 inflammasome forms multiprotein complexes and its sequential assembly is important for its activation. Here, we show that NLRP3 is ...