Negative-sense (NS) RNA viruses deliver into cells a mega-dalton RNA–protein complex competent for transcription. Within this complex, the RNA is protected in a nucleocapsid protein (NP) sheath which the viral polymerase negotiates during RNA synthesis. The NP–RNA templates come as nonsegmented...
不分节段负股RNA病毒(nonsegmented negative-strand RNA viruses,NNSV)属于单负股病毒目,具有许多优良的特性,可以作为活病毒疫苗的候选载体,利用反向遗传学系统,表达外源基因,研发新型疫苗。3) negative-strand RNA viruses 负链RNA病毒4) single strand plus RNA virus 单股正链RNA病毒 1. The results of many...
A negative-stranded RNA virus is a single-stranded RNA virus that is negative-sense. Single-stranded means that the genome is found on a single chain...Become a member and unlock all Study Answers Start today. Try it now Create an account Ask a question Our experts can answer your tou...
Methods for producing infectious, non-segmented, negative-stranded RNA viruses of the Order are provided that involve coexpression of a viral cDNA along with essential viral proteins, N, P, and L in a host cell transiently transfected with an expression vector encoding an RNA polymerase. In alte...
Structure of L-A Virus: A Specialized Compartment for the Transcription and Replication of Double-stranded RNA The genomes of double-stranded (ds)RNA viruses are never exposed to the cytoplasm but are confined to and replicated from a specialized protein-bound compa... Caston,J. R. - 《Journa...
An evaluation of human recombinant interferon beta (rIFN-β) against selected positive- and negative-stranded RNA viruses using an MTT assay procedure : J.J. Kirsi1, W.M. Shannon1, T.P. Monath2, and J. W. Huggins2. Southern Research Institute, Birmingham, AL 35255 USA1, and U.S. ...
(2006). Double-stranded RNA is produced by positive-strand RNA viruses and DNA viruses but not in detectable amounts by negative-strand RNA viruses. J. Virol. 80, 5059-5064.Double-stranded RNA is produced by positive-strand RNA ... V Wagner,F Weber,SB Rasmussen,... - 《Journal of Vir...
The template of the polymerases of negative-stranded RNA viruses, however, is exclusively a ribonucleoprotein complex (RNP) or 'nucleocapsid', consisting of the genomic RNA tightly encapsidated within nucleoprotein (N or NP) and associated with a phosphoprotein (P)....
The kinetics of ratios of positive strands to negative strands (+RNA/-RNA) in vivo in the liver, kidney and spleen were similar, which demonstrated that the replication dynamics were similar in the three organs. 55 single cell samples out of 187 were positive by both positive strands qPCR ...
Plant viruses of the recently recognized family Amalgaviridae have monopartite double-stranded (ds) RNA genomes and encode two proteins an RNA-dependent RNA polymerase (RdRp) and a putative capsid protein (CP). Whereas the RdRp of amalgaviruses has been found to be most closely related to the...