IODIDE peroxidaseHYPOTHYROIDISMLEVOTHYROXINELOW birth weightIMMUNOGLOBULINSThis article explores the impact of the timing of levothyroxine therapy on the neurological development of babies born to women with gestational subclinical hypothyroidism (SCH) and negative thyroid peroxidase antibodies (TPOAbs). ...
. PVDF membranes were blocked with 5% milk, probed with specific primary antibodies followed by peroxidase-conjugated secondary antibodies (GE Healthcare UK Ltd, Little Chalfont, U.K.) and visualized using Western Lightning® Plus-ECL (PerkinElmer, LAS Inc., Shelton, CT, U.S.A.) and x-...
Employing the same purification protocol and surface-biotinylated cells, immunoblots probed with streptavidin-horse radish peroxidase (HRP) or polyclonal antibodies demonstrated the presence of GRP78 and more prominently of ERp5 on the surface of U266 but not U937 cells, which was confirmed by ...
Cell lysates were loaded onto a 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis gel and stained with mouse Flag epitope (Sigma-Aldrich) and anti-HA epitope (Applied Biological Materials) primary antibodies and a horseradish peroxidase- conjugated goat anti-mouse secondary IgG antibody (...
(60,004–1-Ig, ProteinTech) overnight at 4 °C. The cell membranes were incubated with Affinipure Goat Anti-Mouse IgG (SA00001-1, ProteinTech) or Affinipure Goat Anti-Rabbit IgG (SA00001-2, ProteinTech) secondary antibodies conjugated with horseradish peroxidase (HRP) for 1 h at room ...
antibodies (1/1,000 dilution) overnight at 4 °C with agitation. All primary antibodies were resuspended in a solution of TBS containing 3% BSA and 0.1% sodium azide (Sigma-Aldrich). Finally, the PVDF membranes were incubated for 1 h with horseradish peroxidase-conjugated donkey anti-rabbit ...
antibodies at the dilutions recommended by the suppliers and incubated overnight at 4 °C. Next, the membrane was incubated with horseradish peroxidase (HRP)-conjugated secondary antibody, and the bands were visualized using enhanced chemiluminescence substrate (ThermoFisher, 32106). The primary ...
The trans- blotted membrane was incubated with anti-ETV1 rabbit polyclonal antibodies (1:1000; ABCAM, USA) and anti- COP1 mouse monoclonal antibody (1:1000; ABCAM, USA) at 4°C overnight, respectively. Expression of ETV1 or COP1 was detected by horseradish peroxidase (HRP) con- jugated...
primary antibodies [LC3, Cleaved Caspase 3, PTEN, p27, pAkt (Cell Signaling); Bip, CHOP, p62 (Santa Cruz Biotechnology); Ubiquitin (Stressgen); Actin and p21 (Epitomics); Tubulin (Sigma); GAPDH (R&D Laboratories)] and peroxidase-conjugated suitable secondary antibodies (Jackson Immunoresearch)...
Subsequently, horseradish peroxidase conjugated anti-mouse antibody (GE Healthcare Biosciences, Pittsburgh, PA, USA) diluted 1:1500 in blocking solution was added and plates were incubated for 45 min at RT. Receptor levels were detected by addition of 80 μl/well of DPBS supplemented ...