PTH parathyroid hormone ECM extracellular matrix Introduction Fractures of the skeleton, especially at the hip, represent devastating injuries, resulting in disability, increased mortality and high treatment costs. The risk of hip fractures increases with age and, as a consequence of the aging human po...
To evaluate the protein expression of cell lines listed in Additional file 2: Table S2, collected cell pellets were lysed using RIPA buffer (Santa Cruz Biotechnology Inc). The concentration of total protein was determined by the Bradford colorimetric assay (Thermo Scientific, MA). Proteins from ce...
For last 15 years, breast cancer has been classified based on the expression of certain hormone receptors, namely progesterone receptor (PR), estrogen receptor (ER) and anti–human epidermal growth factor receptor 2 (HER2). Almost one and a half decades back, a new term was coined, namely ...
Indeed, the absence of hormone-dependent activity might be explained by the lack of ERα transcriptional co-factors or by an epigenetic landscape in which classical ERα-dependent enhancers are not accessible. Here, we uncovered that ERα downstream targets are increased upon PLK1 inhibition, ...
(B) Western blot analysis of hormone receptor positive cell lines (MCF7 and T47D) and basal-like TNBC cell lines (MDA-MB-468, HCC-3153 and HCC-1806) treated with Dasatinib at the IC50 for 72 h, relative to the control treatment (DMSO). Membranes were immunoblotted for pSMAD2/3, ...
Cell pellets and/or tumor samples were lysed in RIPA buffer (10 mM Tris-HCl pH 8, 140 mM NaCl, 0.1% SDS, 1% Triton X-100, 0.1% sodium deoxycholate, 1 mM EDTA, 0.5 mM EGTA), with 1 mM PMSF (Thermo Fisher, UK, Cat. 78440) added fresh prior to use. The lysed pellets were ...
Exosomes (Exo) hold great promise as endogenous nanocarriers that can deliver biological information between cells. However, Exo are limited in terms of their abilities to target specific recipient cell types. We developed a strategy to isolate Exo exhib
Immunotherapy has a number of advantages over traditional anti-tumor therapy but can cause severe adverse reactions due to an overactive immune system. In contrast, a novel metabolic treatment approach can induce metabolic vulnerability through multiple
(Tarapore et al, 2010), sensitise castration-resistant prostate cancer cells to antihormone therapy (Siddique et al, 2011), induce apoptosis in head and neck squamous cell carcinoma by suppressing NF-κB activation (Lee et al, 2007), inhibit the self-renewal ability of liver tumour-initiating...
Indeed, the lack of estrogen, progesterone and HER2 receptors expression in these tumor cells makes them insensitive to targeted anti-hormone and/or anti-HER2 therapies1. As a consequence, the main treatment remains conventional chemotherapy, with good initial response in some cases, but the tumor...