This process was repeated for a total of three cycles. Phenylmethanesulfonyl fluoride (PMSF; Fude, Zhejiang, PRC) was added to the RIPA lysis buffer at a concentration of 1:100. After extraction, the protein concentration was measured using a BCA assay kit (Glpbio, California, USA), and ...
Interestingly, nitrofen’s toxicity is much lower than many agents currently used for the treatment of BrCa which are associated with serious adverse health effects at therapeutic doses19,20,21. This project has grown out of an earlier study that examined the mechanism by which nitrofen induces ...
Breast cancer (BC) is the most commonly diagnosed cancer among women and poses a significant threat to their health. According to the latest worldwide cancer statistics of 2020, BC accounted for the highest number of new cases (2.26 million) among cancers, surpassing lung cancer (2.2 million) ...
Proteins from cell lysates were separated in 10% sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) and subsequently transferred to a polyvinylidene fluoride (PVDF) membrane (GE Healthcare Technologies, Vienna, Austria) with a semi-dry blotter. The membranes were blocked using 5% ...
described.49 Briefly, cells were cross-linked with 1% formaldehyde for 10 min, quenched with 0.125 M glycine, and lysed on ice for 10 min in cell-swelling buffer containing 5 mM Pipes (pH 8.0), 85 mM KCl, 0.5% NP-40, 0.5 mM phenylmethylsulfonyl fluoride, and 100 ng/ml leupeptin and...
The zero-inflated negative binomial regression model (ZINB) is often employed in diverse fields such as dentistry, health care utilization, highway safety, and medicine to examine relationships between exposures of interest and overdispersed count outcomes exhibiting many zeros. The regression coefficients...
According to a report released by the World Health Organization in 2020, the number of breast cancer (BC) cases is on the rise worldwide. It is estimated that there are 2.3 million new breast cancer cases worldwide, accounting for 11.7% of the world’s new cases. As the malignant tumor...
Following protein estima- tion, 20 μg of each protein sample was loaded onto a 10% sodium dodecyl sul- fate–polyacrylamide gel electrophoresis gel, transferred onto a polyvinylidene di- fluoride membrane and immunolabeled for either phosphorylated ERK1/2 (anti- rabbit, 1:2000; Cell Signaling)...
The impair- ment of myogenic differentiation induced by increased UCP2 levels13 also suggests its contribution to cellular dedifferen- tiation during tumor evolution. Despite known physiological functions of UCP2, key regulatory pathways associated with its expression in human health and disease remain ...
Membranes were incubated either overnight or for 40 h at 4 °C with primary antibodies and exposed to secondary HRP-coupled anti-mouse (Merck, cat# GENA931) or anti-rabbit (Merck, cat# GENA934) antibodies (1:5 000–10 000, GE Healthcare) either for 2 h at RT or overnight ...