Furthermore, experimen- tally introduced UCP2 led to a striking neutralization of TGFb effects on cell morphology and growth in responsive tumor cells (Figure 2c). For further evaluation of UCP2 regulation by TGFb/SMAD, the 3 kb human UCP2 promoter was analyzed by Vector NTI Suite software ...
2C–F), we next investigated the potential functional role of plectin in mediating the effects of dual HD-PTEN-loss by introducing plectin knock-out (PLEC-KO) into PC3-α6-KO and PC3-β4-KO cells. Depletion of plectin efficiently prevented upregulation of FA-mediated signaling (Fig. 4A...
In vivo PTT treatment effects on TNBC tumors and corresponding systematic toxicity were evaluated. Results Nectin-4 is overexpressed in MDA-MB-468 TNBC cells, which could specifically uptake 99mTc-HYNIC-mAbNectin-4 with high targeting in vitro. The corresponding immunoSPECT imaging demonstrated ...
A target of 15 μg of protein per well was used for SDS-PAGE. Following transfer, polyvinylidene fluoride (PVDF) membranes were blocked overnight at 4 °C in Lindsay et al. Clinical Epigenetics (2017) 9:95 Page 3 of 13 Fig. 1 Drug effects on H3K27me3 levels vary between cell line...
To isolate the insoluble protein fraction containing LRRc17-Fc, cells were resuspended in lysis buffer (50 mm Tris-Cl (pH 8.0), 100 mm NaCl, 5 mm EDTA, 0.1 mm phenylmethylsulfonyl fluoride, 1 mm dithiothreitol, and 0.5% Triton X-100), ultrasonicated at 150 watts for 15 min, and ...
Given the 72% homology between Rab27a and Rab27b, it is possible that the dominant negative effects of overexpressed Rab27b mutants arise from other intracellular processes, perhaps indirectly through consumption of Rab27a binding proteins functioning in the Rab cycle. It is more likely, however,...
described.49 Briefly, cells were cross-linked with 1% formaldehyde for 10 min, quenched with 0.125 M glycine, and lysed on ice for 10 min in cell-swelling buffer containing 5 mM Pipes (pH 8.0), 85 mM KCl, 0.5% NP-40, 0.5 mM phenylmethylsulfonyl fluoride, and 100 ng/ml leupeptin and...
Invasive breast cancer (BrCa) is predicted to affect 1 in 9 women in a lifetime;1 in 32 will die from this disease. The most aggressive forms of BrCa, basal-like/triple-negative phenotype (TNBC), are challenging to treat and result in higher mortality du
Thus, modulation of internalization could be a general mechanism regulating the activity of transmembrane metalloproteases toward their substrates. Several laboratories have examined the effects of kinase activation on ADAM trafficking and protease activity. PMA caused down-regulation of ADAM17/TACE surface ...
REP1 is part of the Rab geranylgeranylation machinery, a modification that is essential for Rab function in membrane traffic. The loss of REP1 in CHM patients may trigger retinal degeneration through its effects on Rab proteins. We have previously reported that Rab27a is the Rab most affected ...