Potency was abolished by either replacement of the nitrogen atom by a carbon atom (2) or introduction of a carbon–carbon double bond in place of the amino-methyl linker (3) (Table 2). A more favorable replacem
Figure 1. Mutations in OTULIN in Patients with a Systemic Autoinflammatory Syndrome (A) Segregation of the inflammatory symptoms (filled symbols) and the c.815T>C substitution in OTULIN in the affected kindred. ○, females; □, males; double lines, consanguineous relationship; crossed symbols, de...
Peptides (4 μg) were added (50 s) to liposomes composed of negatively charged phosphatidylglycerol (solid line) or zwitterionic phosphatidylcholine (dotted line) double-labeled with NBD-PE and Rh-PE. An intercalation of the peptides led to an increase of the FRET signal (IDonor/IAcceptor)....
In addition, 2 is bound to the LP site through a water-mediated bridge between a secondary amine on 2 and the Ser345 side chain for one chain within the asymmetric unit and an H-bond between a sulfonamide oxygen to the backbone nitrogen of Cys380 (Figure 1G). While both molecules bind...