EDTA blood samples were obtained from three patients (family 2–4, III:1) and one unaffected mutation carrier (family 4, II:2). PGRN plasma levels were measured using a specific ELISA kit (Human Progranulin kit; Adipogene, Korea). A cut-off value of 61 ng/mL is considered the threshold...
Changes in blood volume will lead to changes in bioimpedance. This study aims to study changes in upper (UL) and lower (LL) extremities bioimpedance during LBNP-induced hypovolemia. Methods: This was a prospective observational study of healthy volunteers who underwent gradual...
A minimum of 100 ng of total RNA was analyzed on the nCounter platform46 (Nanostring Technologies, Seattle, USA) using a 192-gene custom panel47, which includes the 50 genes of the PAM50 test, the 27 genes of the HER2DX assay47, immune-related genes, breast cancer-related genes ...
Somewhat atypical for FA was his strong learning disability associated with an intelligence quotient test score of about 70. Cellular tests using blood lymphocytes and SV40-transformed fibroblast cell lines established at age 5 years, confirmed the hypersensitivity to DNA cross-linkers (Fig. 1a,b)....
Bottom panel: correlations between the copy number values of 1q32.1 and the abundances of uridine and D-pantethine. SCNA-related metabolites were shown as lines, and samples were ordered by increasing copy number values. The abundances of the metabolites were illustrated in colors. (e) Heatmap...
Ficoll-Hypaque gradient centrifugation was used to separ- ate peripheral blood mononuclear cells (PBMCs) at 400×g for 30 min at 20 C. PBMCs were seeded on precoated IFN-γ ELISpot plates and incubated with media con- taining peptide antigens derived from ESAT-6 (labeled panel A) or ...
Buparlisib is one of the PI3K inhibitors with highest blood-brain barrier penetration [23]. However, the physiopathology of mood disorders associated with pan-PI3K inhibitors has not been well characterized, and it remains unclear whether the higher incidence of neuropsychiatric effects observed with...
expression of immune-related genes is dependent on IFN-β exposure, we treated two HPV-negative HNSCC cell lines (HN-6, HN-SCC-151) with negative control or SMYD3-specific siRNAs for 72 h with or without exposure to IFN-β and conducted qPCR for a panel of representative immune-related ...
A minimum of 100 ng of total RNA was analyzed on the nCounter platform46 (Nanostring Technologies, Seattle, USA) using a 192-gene custom panel47, which includes the 50 genes of the PAM50 test, the 27 genes of the HER2DX assay47, immune-related genes, breast cancer-related genes ...
correlations between the copy number values of 8q23.3 and the abundances of deoxyinosine, ribothymidine, uracil and some amino acids. Bottom panel: correlations between the copy number values of 1q32.1 and the abundances of uridine and D-pantethine. SCNA-related metabolites were shown as lines,...