LentiCRISPRv2bleo-Nectin-2 plasmid质粒载体-BioVector NTCC典型培养物保藏中心Plasmid,Name质粒名称:,,,LentiCRISPRv2bleo-Nectin-2Gene/Insert插入基因名称:,,,A,gRNA,targeting,the,mouse,Nectin-2,gene.Cat#货号:,BioVector-0170295Map图谱:Sequence序列:Contact
根据Nectin-4基因序列设计特异性引物,进行PCR扩增,将其克隆至原核表达载体pGEX-4T-1中,经PCR,酶切和测序鉴定,成功构建了重组表达质粒pGEX-4T-Nectin-4.将重组质粒转化大肠埃希氏菌Rosetta感受态细胞,经IPTG诱导表达目的蛋白.表达产物经SDS-PAGE和Western blot鉴定正确后,纯化后免疫BALB/c小鼠,制备多克隆抗体血清,并利...