NEBNext Ultra II Q5 预混液(NEB #M0544)现已商售,它能够进一步提高文库扩增的一致性,即使在 GC 富集区域依然表现优异。 这两种预混液中均含有 Q5 超保真 DNA 聚合酶,它是一种具备热稳定性的新型 DNA 聚合酶,具有 3´→5´ 核酸外切酶活性,并且融合了具有增强持续合成能力的 Sso7d 结构域。Q5 还具有...
In this application note, we describe the automation of the NEBNext(R) UltraTM DNA Library Prep Kit for Illumina(R) on a Hamilton Microlab STARletinstrument. The design allows a capacity of 8-48 samples per run. Ready to sequence libraries with highyield and quality can be obtained from ...