A. Poly(A)-containing mRNA was isolated from Universal Human Reference RNA (UHRR) (Agilent®), using the NEBNext®Poly(A) mRNA Magnetic Isolation Module (NEB #E7490). Libraries were prepared using the NEBNext UltraExpress™ RNA Library Prep Kit, Kapa mRNA HyperPrep® Kit or Illumina...
试剂盒内每个组份都通过严格的质量监控,每套试剂都经过文库制备,并通过 Illumina 测序平台测序验证。 NEBNext Ultra™ RNA 可从不同起始量的转录本展现均一的序列覆盖度 按照NEBNext Ultra™ RNA 操作说明,使用 NEBNext Poly(A) mRNA 磁性分离模块(NEB #E7490),以 10 ng、100 ng 或 1 μg 总 RNA(通用...
NEBNext® Ultra™II RNA 文库制备试剂盒 NEBNext Ultra II DNA Library Prep Kit for Illumina 详细描述: NEBNext Ultra II RNA 文库制备试剂盒能够用更低起始量的 RNA 构建高质量的文库。文库制备每一个步骤中的试剂都经过优化,。新一代的 NEBNext 试剂采用了快速、流程化、自动化的工作流程,在降低 PCR...
The NEBNext®Ultra™ Directional RNA Library Prep Kit for Illumina®contains reagents for preparation of strand-specific RNA libraries for Illumina sequencing. Please note that adaptors, primers, rRNA depletion reagents and poly(A) mRNA isolation reagents are not included in the kit and are av...
使用NEBNext单细胞/超低起始量 RNA 文库制备试剂盒建库,能显著增加转录本检测数量。以 Jurkat 单细胞(6个重复)为样本制备文库,分别采用 NEBNext单细胞/超低起始量 RNA 文库制备试剂盒、SMART-Seq v4 Ultra Low Input RNA 试剂盒(Clontech #634891)及Nextera XT DNA Library Prep 试剂盒(Illumina #FC-131-1096...
Kit Type Nucleic acid assembly/cloning/editing View Product On Supplier's Website Add to Procurement List Sign inorregisterto save this reagent to your favourites Supplier provided information Validations None provided Reactivity None provided
Ultra II DNA 和 Ultra II FS DNA 试剂盒均有包含或不含 SPRIselect® 磁珠的包装。 用于Illumina 测序平台的 NEBNext 试剂:DNA 文库制备 除了每种组份都经过严格的质量监控以外,NEBNext DNA 试剂盒还都经过构建基因组 DNA 文库或 ChIP-Seq 文库,再在 Illumina 测序平台测序进行验证。NEBNext 试剂盒里面,各个...
–RNA Sample Preparation –Second Strand Synthesis of cDNA 概述 The NEBNext Ultra II Non-Directional RNA Second Strand Synthesis Module has been optimized to generate double-stranded cDNA from first-strand cDNA, as part of the NEBNext non-directional RNA library preparation workflow. The NEBNext ...
The NEBNext Multiplex Small RNA Library Prep Kit for Illumina (Index Primers 1-48) contains the adaptors, primers, enzymes and buffers required to convert small RNAs into indexed libraries for next-generation sequencing on the Illumina platform. The novel workflow has been optimized to minimize ...
also included in all of the NEBNext Ultra II library prep kits, for DNA and RNA. NEBNext Ultra II Q5 Master Mix provides improved coverage of known low coverage regions of the human genome. Libraries were prepared from Human NA19240 genomic DNA. One library was not amplified. The other ...