How many cycles of PCR should I perform with NEBNext Ultra II Q5 Master Mix? Can I use NEBNext Ultra II Q5 Master Mix to amplify low quality DNA, such as FFPE DNA? Will NEBNext Ultra II Q5 Master Mix incorporate dUTPs? Can I use NEBNext Ultra II Q5 Master Mix to amplify bisulfite...
NEBNext® Q5 热启动超保真 PCR 预混液是独创的 Q5 DNA 聚合酶热启动 NEBNext 剂型。在所有的 NEBNext Ultra 试剂盒中,均含有此剂型,用于 DNA 和 RNA 文库制备。 NEBNext Ultra II Q5 预混液(NEB #M0544)现已商售,它能够进一步提高文库扩增的一致性,即使在 GC 富集区域依然表现优异。 这两种预混液...
How many cycles of PCR should I perform with the NEBNext Q5U Master Mix? Are the DNA products produced by the NEBNext Q5U Master Mix blunt-ended or do they have a single base 3´ overhang? Will NEBNext Ultra II Q5 Master Mix incorporate dUTPs?
NEBNext Ultra II Q5 预混液是 Q5 DNA 聚合酶的新版本,针对二代测序(NGS)文库的高保真稳定扩增进行了优化。进一步提高了文库扩增的一致性,包括 GC 富集区域。这个预混液中的酶是 Q5 超保真聚合酶,它是一个非常新颖的热稳定的 DNA 聚合酶,有 3´ →5´ 外切酶活性,并且融合了一个可以增强持续合成能力的...
NEBNext® Ultra II Q5® Master Mix E7649AAVIAL -20 2 x 1.2 ml Not Applicable 特性和用法 需要但不提供的材料 80% 乙醇(新鲜配制) 无核酶水 0.1X TE(1 mM Tris-HCl,pH 8.0,0.1 mM EDTA) DNA LoBind 管(Eppendorf #022431021) NEBNext 单样本或多样本接头引物试剂盒(NEB #E7350、#E7335、...
NEBQ5® 高保真 DNA 聚合酶产品详细列表: 货号 产品 规格 品牌 M0541L NEBNext® High-Fidelity 2X PCR Master Mix 250 reactions NEB M0543L NEBNext® Q5® Hot Start HiFi PCR Master Mix 250 reactions NEB M0544L NEBNext® Ultra™ II Q5® Master Mix ...
–NEBNext Ultra II RNA First Strand Synthesis Module (NEB #E7771) –NEBNext Ultra II End Repair/dA-Tailing Module (NEB #E7546) –NEBNext Ultra II Ligation Module (NEB #E7595) –NEBNext Ultra™ II Q5® Master Mix (NEB #M0544) –NEBNext® Oligos for Illumina® (NEB #E7335,...
For Illumina library construction, the NEBNext Ultra II End Repair/dA-Tailing Module is designed for use with the following: • NEBNext Ultra II Ligation Module (NEB#E7595) • NEBNext Ultra II Q5®Master Mix (NEB#M0544) • NEBNext Oligos for Illumina(NEB#E7335,#E7500,#E7710,#E...
NEBNext Ultra II Q5 Master Mix provides uniform GC coverage for microbial genomic DNA with a broad range of GC composition. Libraries were made using 100 ng of the genomic DNAs shown and the NEBNext Ultra II DNA Library Prep Kit. Libraries were amplified using the NEBNext Ultra II Q5 Mast...
NEBNext®Ultra™ II Ligation Module (NEB #E7595) or NEBNext®Ultra Ligation Module (NEB #E7445) NEBNext®Ultra™ II Q5® Master Mix (NEB #M0544) or NEBNext®High Fidelity 2X PCR Master Mix (NEB #M0541) NEBNext®Singleplex or Multiplex Oligos for Illumina®(NEB #E7350...