Is NEBNext®High-Fidelity 2X PCR Master Mix the same as the Q5®High-Fidelity Master Mix? Do I need to make any changes to the cycling conditions for my NGS library amplification I have previously been using with Phusion High-Fidelity PCR Master Mix? How many cycles of PCR should I p...
The V3 ARTIC primers have been balanced, using methodology developed at NEB based on empirical data from sequencing. In combination with optimized reagents for RT-PCR, the kits deliver improved uniformity of amplicon yields from gRNA across a wide copy number range. The Express protocol removes th...