NEBNext® FFPE DNA Repair Mix M6630LVIAL -20 1 x 0.192 ml Not Applicable NEBNext FFPE DNA Repair Buffer E6622AAVIAL -20 1 x 0.624 ml Not Applicable 特性和用法 需要但不提供的材料 建库试剂(例如:NEBNext Ultra II DNA 文库制备试剂盒,NEB #E7645) 100% 乙醇 无核酶水 10 mM Tris-HCl(pH...
NEBNext FFPE DNA 修复混合液是由多种酶按照一定的配方混合而成,经过优化和验证可以用来修复 FFPE 样本 DNA。FFPE DNA 修复混合液能提高文库产量,没有偏嗜性,从总体上提高建库成功率。 表格1:FFPE DNA 的损伤类型及 NEBNext FFPE DNA 修复混合液的修复能力 图1:FFPE DNA 修复混合液对文库产量的影响。在构建文...
What size should I fragment my DNA to? If my FFPE DNA is fragmented, should I do a shearing step? Can I repair my FFPE DNA using the NEBNext FFPE DNA Repair Mix (NEB #M6630)? How can I warm Bead Wash 1 (BW1) to dissolve any precipitate? If I have adaptor dimer in my finishe...
Can I use the FFPE DNA Repair Mix to repair other types of low quality DNA other than FFPE DNA? What is the difference between the NEBNext FFPE DNA Repair v2 Module (NEB #E7360) and the NEBNext FFPE DNA Repair Mix (NEB #M6630)?