v2.5.2 NEBaseChanger assists in the design of primers for site-directed mutagenesis experiments using the Q5 Site-Directed Mutagenesis Kit. Additional resources for NEBaseChanger, including related products, videos, tutorials, and application notes are availablehere. ...
NEBaseChanger can design primers specific to the mutagenesis experiment you are performing and calculate a recommended custom annealing temperature.
在squence窗口输入DNA序列,去掉无法识别的部分,对序列进行重命名 选择需要定点修饰的方式,选择定点修饰位点,修改为需要的序列