How can I improve on a low yield of RNA from the transcription reaction? Are modified nucleotides included in the kit? Do I need to add DTT to the reaction? 问题解决指南 对照反应FLuc 对照模板 DNA 是一种线性化质粒,包含受 T7 启动子调控的萤火虫荧光素酶基因。目标转录产物的大小为 1.8 kb。
The HiScribe T7 High Yield RNA Synthesis Kit is an extremely flexible system forin vitrotranscription of RNA using T7 RNA Polymerase. The kit allows for synthesis many kinds of RNA including internally labeled and co-transcriptionally capped transcripts. RNA synthesized from the kit is suitable for...
Updated to allow forin vitrotranscription with both SP6 and T7 promoters BsaI-site removed from the ampicillin-resistant gene (allows for the cloning of Golden Gate Assembly modules) Fast cloning experiments with 5-minute ligation step Simplified screening with low/no colony background and no blue...
DNA Template Preparation: Linearized plasmid dNa, pCr products or synthetic dNa oligonucleotides can be used as templates for in vitro transcription with the HiScribe T7 Quick High Yield rNa Synthesis Kit, provided that they contain a double-stranded T7 pro- moter region upstream of the sequence ...
Figure 1. Transcription by T7 RNA Po ymerase Introduction: The HiSc ibe T7 Quick High Yiel rNa Synthesis Kit is esigne f quick set- u n ucti n f l ge m unts f rNa in vitro. The e cti n c n be set u c nveniently by c mbining the NTp buffe mix, T7 rNa p lyme se...
• T7 RNA 聚合酶促成体外转录 • 最低的核酸酶和蛋白酶活性,以保证合成蛋白和编码靶标的稳定性 • 模板可以是质粒 DNA、线性 DNA 或 mRNA • 目标蛋白可以在几个小时内合成并可见 • 合成多种靶标肽和蛋白 • 合成的蛋白可进行共翻译放射性标记或荧光标记 ...
Featured Products HiScribe® T7 mRNA 合成试剂盒(含 CleanCap® Reagent AG) HiScribe® T7 高效 RNA 合成试剂盒 Faustovirus 加帽酶 牛痘病毒加帽系统 mRNA 帽结构 2'-O-甲基转移酶 EnGen® sgRNA 合成试剂盒,S. pyogenes DNase I-XTSubmit...
e2040-hiscribe-t7-high-yield-rna-synthesis-kit SP6 RNA 聚合酶 m0251-t7-rna-polymerase rNTP 混合液 小鼠RNase 抑制剂 m0307-rnase-inhibitor-human-placenta 注意事项 NEB PCR 克隆试剂盒包含的试剂足以进行 20 次 10 μl 的克隆反应。试剂盒还提供引物,便于进行下游菌落 PCR 筛选和/或测序。
但对 RNase 1、RNase T1、S1 核酸酶、RNase H 或来源于曲霉属(Aspergillus)的 RNase 无效。此外,与下列聚合酶共同使用时,未观察到 RNase 抑制剂抑制聚合酶的活性,如:TaqDNA 聚合酶、AMV 或 M-MuLV 反转录酶或噬菌体 RNA 聚合酶(SP6、T7 或 T3)。
The HiScribe T7 mRNA Kit with CleanCap Reagent AG utilizes an optimized RNA synthesis formulation and trinucleotide cap analog technology for co-transcriptionally capping mRNAs that contain a natural Cap-1 structure in a single simplified reaction without compromising RNA yield. By using a DNA ...