T7 RNA Polymerase Mix M0255LVIAL -20 1 x 0.5 ml Not Applicable LiCl Solution B2051AAVIAL -20 1 x 6.5 ml Not Applicable NTP Buffer Mix N2052AAVIAL -20 2 x 1.25 ml Not Applicable特性和用法 需要但不提供的材料 DNA 模板 热循环仪或 37℃ 温育器。 无核酸酶水 缓冲液或水饱和苯酚/氯仿 乙...
T7 RNA Polymerase in the lac operon - no λ prophage Tight control of expression by laclq allows potentially toxic genes to be cloned Control of T7 RNA Polymerase by lysozyme allows potentially toxic genes to be expressed Deficient in proteases Lon and OmpT ...
T7 RNA Polymerase in the lac operon - no λ prophage Control of T7 RNA Polymerase by lysozyme allows potentially toxic genes to be expressed Deficient in proteases Lon and OmpT Resistant to phage T1 (fhuA2) Free of animal products
The HiScribe T7 High Yield RNA Synthesis Kit is an extremely flexible system forin vitrotranscription of RNA using T7 RNA Polymerase. The kit allows for synthesis many kinds of RNA including internally labeled and co-transcriptionally capped transcripts. RNA synthesized from the kit is suitable for...
• T7 RNA 聚合酶促成体外转录 • 最低的核酸酶和蛋白酶活性,以保证合成蛋白和编码靶标的稳定性 • 模板可以是质粒 DNA、线性 DNA 或 mRNA • 目标蛋白可以在几个小时内合成并可见 • 合成多种靶标肽和蛋白 • 合成的蛋白可进行共翻译放射性标记或荧光标记 • 蛋白可进行反向纯化,或直接进行...
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T3 RNA Polymerase M0378S 5,000 units ￥799 ¥599 T7 RNA Polymerase M0251L 25,000 units ￥2,789 ¥2,092 M0251S 5,000 units ￥699 ¥524 SP6 RNA Polymerase M0207L 10,000 units ￥2,789 ¥2,092 M0207S 2,000 units ￥699 ¥524 HiScribe T7 High Yield RNA Synthesis...
2. assemble the reaction at room temperature in the following order: Nuclease-free water X μl NTp Buffer Mix 10 μl 10 mM each NTp final Template dNa X μl 1 μg T7 rNa polymerase Mix 2 μl Total reaction volume 20 μl 3. Mix thoroughly and pulse-spin in a microfuge. Incubate ...
Can I use a different concentration or formulation of T7 RNA Polymerase in my NEBExpress Cell-freeE. coliProtein Synthesis reactions? What size is the NEBExpress®Control DHFR-His Plasmid? The protocol recommends shaking the reactions vigorously. Is this necessary?
使用NEB 高纯 RNA 聚合酶,灵活建立反应体系 NEB 提供多种 RNA 聚合酶,可用于体外转录实验。例如 T3, T7, SP6, and Hi-T7 RNA 聚合酶,其中 T7 和 Hi-T7 RNA 聚合酶提供高浓度剂型(为标准浓度的 20 倍),是希望自己建立和优化体外转录反应体系的有经验用户的理想选择。