T7 RNA Polymerase Mix M0255LVIAL -20 1 x 0.5 ml Not Applicable LiCl Solution B2051AAVIAL -20 1 x 6.5 ml Not Applicable NTP Buffer Mix N2052AAVIAL -20 2 x 1.25 ml Not Applicable特性和用法 需要但不提供的材料 DNA 模板 热循环仪或 37℃ 温育器。 无核酸酶水 缓冲液或水饱和苯酚/氯仿 乙...
T7 RNA Polymerase M0251S 5,000 units Hi-T7 RNA Polymerase M0658S 5,000 units E.coli Poly (A) Polymerase M0276L 500 units E.coli Poly (A) Polymerase M0276S 100 units Vaccinia Capping System M2080S 400 units mRNA Cap2'-O-Methyltransferase M0366S 2,000 units 3'-O-Me-m7G(5')pp...
An E.coli strain that carries a plasmid encoding the engineered T7 RNA Polymerase gene. Storage Condition -20±5℃ Quality Control Activity ≥ 50ku/ml Purity (SEC-HPLC) ≥95% Nonspecific nuclease residue negative Endonuclease residue negative ...
通过小规模优化体系(20 µl 体系),发现 0.1 单位的无机焦磷酸酶(E.coli)与 20 单位的 RNase 抑制剂(最终浓度分别为 0.005 U/µl 和 1 U/µl)组合使用,能够显著提高 RNA 产量,并且这一方法具有可扩展性(见图 2 B)。 图2:T7 RNA Polymerase titration indicates enzyme amount required to reach m...
T7 表达 lysY/IE. coli 感受态细胞(高效级) High efficiency chemically competentE. colicells suitable for T7 expression with highest level of expression control. T7 RNA Polymerase in the lac operon - no λ prophage Tight control of expression by laclq allows potentially toxic genes to be cloned...
Figure 1. Transcription by T7 RNA Polymerase Figure 2. Time course of standard RNA synthesis from three DNA templates Reactions were incubated at 37°C in a PCR machine. Transcripts were purified by spin columns and quantified on NanoDrop™ Spectrophotometer. ...
T7 RNA Polymerase T7 RNA聚合酶 替代NEB M0251S NEB M0251L Invitrogen 18033019 Invitrogen 18033100 Thermo EP0111 Thermo EP0112 Efficient transcription of mRNA of different lengths Fig. 1 Three plasmids (lane 1 with poly A tail, lane 2 and 3 without poly A tail) were used as templates, ...
T7 RNA PolymeraseM0251L25,000 units￥2,789¥2,092 M0251S5,000 units￥699¥524 SP6 RNA PolymeraseM0207L10,000 units￥2,789¥2,092 M0207S2,000 units￥699¥524 HiScribe T7 High Yield RNA Synthesis KitZ1925S50 rxns /10 preps￥2,948¥1,916 ...
T7 RNA Polymerase M0251L 25,000 units ¥2,959 ¥2,367 M0251S 5,000 units ¥739 ¥591 Vaccinia Capping System M2080S 400 units ¥1,509 ¥1,207 mRNA Cap2'-O-Methyltransferase M0366S 2,000 units ¥659 ¥494 Pyrophosphatase, Inorganic (E.coli) M0361L 50 units ¥...
Can I use a different concentration or formulation of T7 RNA Polymerase in my NEBExpress Cell-freeE. coliProtein Synthesis reactions? What size is the NEBExpress®Control DHFR-His Plasmid? The protocol recommends shaking the reactions vigorously. Is this necessary?