随附专为 NEB 10-beta 和 NEB StableE.coli感受态细胞优化的复苏培养基;请勿使用 SOC Outgrowth 培养基。 E. coli化学感受态细胞,适用于高效级转化。 非常适合用于含重复元件和不稳定插入片段的质粒克隆的扩增分离 可用于分离和扩增逆转录病毒/慢病毒克隆 携带endA突变(与 Stbl3 相反),因此
Transformation, High-throughput cloning and automation solutions 产品组分信息 本产品提供以下试剂或组分: NEB #名称组分货号储存温度数量浓度 C3019H-80 NEB®10-beta/Stable Outgrowth MediumB9035SVIAL41 x 25 mlNot Applicable pUC19 VectorN3041AVIAL-201 x 0.025 ml50 pg/µl ...
The kit is shipped on dry ice. Upon arrival, store the competent cells (in the large exterior box) at -80°C, the components in the small interior box at -20°C and the NEB™ 10-beta/Stable Outgrowth Medium at room temperature or 4°C. ...
Fast transformation plating with glass beads In this video, see how transformation plating can be fast and easy using glass beads. How to Perform a Transformation with NEB®Competent Cells Use this protocol for maximum transformation efficiency. ...
随附专为 NEB 10-beta 和 NEB Stable E.coli 感受态细胞优化的复苏培养基;请勿使用 SOC Outgrowth 培养基。 NEB 10-beta E. coli 感受态细胞是广泛使用的 DH10B 的衍生菌株。它具有 T1 噬菌体抗性且属于内切酶 I(endA1)缺陷型,可用于高质量质粒的制备。 高转染效率菌株,是克隆大质粒和 BAC 的理想选择 ...
Transformation Protocol for NEB PCR Cloning Kit Plating Protocol for NEB PCR Cloning Kit Insert Screening Protocols for NEB PCR Cloning Kit RNA Synthesis of Cloned Insert Transcripts 说明书 产品说明书包含产品使用的详细信息、产品配方和质控分析。
Transformation Protocol for Using NEB Golden Gate Assembly Mix (E1600) Recommended Screening Protocols for Using NEB Golden Gate Assembly Mix (E1600) Golden Gate (24 Fragment) Assembly Protocol Golden Gate Assembly Protocol for Using NEBridge®Golden Gate Assembly Kit (BsaI-HF®v2) (E1601) ...
Transform NEB® 5-alpha, NEB Stable or NEB 10-beta Competent E. coli cells (provided in the cloning kit, bundle or purchased separately from NEB) with 2.0 μl (10%) of the chilled assembled product, following the transformation protocol. Related...
NEB Cloning Competent Cells Experience high transformation efficiencies Enjoy compatibility with 96-well and 384-well formats Find the right fit with bulk formats and custom packaging options Choose from: NEB 5-alpha Competent E. coli (High Efficiency) (NEB #C2987) NEB 10-beta Competent E...
allowing you to obtain ultra-high income. In addition, NEB also has the highest and most stable APY in the entire market. No matter from which perspective, DefiNEB must be the next king. I hope everyone can seize the opportunity, seize DefiNEB, and fight for their own reputation and inte...