Do SHuffle cells grow in minimal media? Are SHuffle strains temperature sensitive? Is the genome of SHuffle strains available? Is T7 expression subject to catabolite repression in NEB T7 Express or SHuffle strains? Can one use SHuffle cells to secrete my protein of interest?
在T7 启动子调控下,表达自质粒的恶性疟原虫几丁质酶(PfCHT1)含三个半胱氨酸。诱导后收集细胞并制备粗细胞裂解物。使用显色底物(CalBioChem #474550)测定 PfCHT1 活性,并使用 Bradford 试剂进行蛋白浓度测定。 注意事项 贮存和处理:感受态细胞应在 -80℃ 下贮存。在 -20℃ 贮存会导致转化效率显著降低。当细胞温度...
SHuffle T7 表达 E. coli 感受态细胞 优点 有助于细胞质中蛋白质二硫键的折叠 T7 表达 蛋白酶缺失的 B 菌株 BL21 源增强型菌株 无动物来源产品 概述 T7 表达菌株,在细胞质中正确折叠含有多个二硫键的重组蛋白的能力更强。 基因型 fhuA2 lacZ::T7 gene1 [Ion] ompT ahpC galλatt::pNEB3-r1-cD...
T7 Express Competent E. coli (High Efficiency) C2566HVIAL -80 20 x 0.05 ml Not Applicable C2566I -80 pUC19 Vector N3041AVIAL -20 1 x 0.025 ml 50 pg/µl SOC Outgrowth Medium B9020SVIAL 4 1 x 25 ml Not Applicable T7 Express Competent E. coli (High Efficiency) C2566IVIAL ...
SHuffle Express Protease deficient/B strain Enhanced capacity to correctly fold proteins with multiple disulfide bonds in the cytoplasm 1 x 107 50 B • • • • spec(6), nit Dam+,Dcm–, M.EcoKI– SHuffle T7 Express T7 expression Protease deficient/B strain Enhanced capacity to...
T7 Expression, Toxic Protein Expression, Expression of Difficult Proteins, Peptide Ligation, Protein Expression in E. Coli, Protein Expression 产品组分信息 本产品提供以下试剂或组分: NEB #名称组分货号储存温度数量浓度 C3010I-80 T7 ExpresslysYCompetentE. coli(High Efficiency)C3010IVIAL-806 x 0.2 ml...
T7 ExpresslysY/IqCompetentE. coli(High Efficiency)C3013IVIAL-806 x 0.2 mlNot Applicable 特性和用法 Antibiotic for Plasmid Selection 用于质粒筛选的抗生素使用浓度 Ampicillin100 µg/ml Carbenicillin100 µg/ml Kanamycin30 µg/ml Streptomycin25 µg/ml ...
Express a variety of proteins with our competent cells Our popularBL21andBL21(DE3)CompetentE. colistrains are available for routine expression Lemo21(DE3)CompetentE. colioffers tunable T7 expression for difficult targets For expression of His-tagged proteins, we offerNiCo21(DE3)CompetentE. coli ...
NEB C3028H SHuffle Express Competent E. coli 6 x 0.05 ml ¥789 NEB C3029H SHuffle T7 Express Competent E. coli 6 x 0.05 ml ¥789 NEB C3030H SHuffle T7 Express lysY Competent E. coli 6 x 0.05 ml ¥789 NEB C3037I NEB Express Iq Competent E. coli (High Efficiency) 6 x ...
NEB C3030H SHuffle T7 Express lysY Competent E. coli 6 x 0.05 ml ¥789 NEB C3037I NEB Express Iq Competent E. coli (High Efficiency) 6 x 0.2 ml ¥1,699 NEB C3040H NEB Stable Competent E. coli (High Efficiency) 20 x 0.05 ml ¥3,599 NEB C3040I NEB Stable Competent E...