preferably with a restriction enzyme that leaves blunt or 5´ overhangs at the 3´ end of the template. These are favorable for proper run-off transcription by T7 RNA Polymerase (NEB #M0274), while 3´ overhangs may result in unwanted transcription products. To avoid adding extra nucleot...
Controlled temperature coefficient Excellent high frequency characteristics Exceptionally low noise; typically 0.10 μV/V Low voltage coefficient to ± 5 ppm/V Special tolerance and or TC matching available on request • Material categorization: for definitions of compliance please see www.vishay....
Controlled temperature coefficient Excellent high frequency characteristics Exceptionally low noise; typically 0.10 μV/V Low voltage coefficient to ± 5 ppm/V Special tolerance and or TC matching available on request • Material categorization: for definitions of compliance please see www.vishay....
If the plasmid dNa template is contaminated with rNase, perform phenol:chloroform extraction, then ethanol precipitate and dis- solve the dNa in nuclease-free water (see template dNa preparation section). 12 RNA Transcript of larger Size than Expected If the rNa transcript appears larger than ...
Controlled temperature coefficient Excellent high frequency characteristics Exceptionally low noise; typically 0.10 μV/V Low voltage coefficient to ± 5 ppm/V Special tolerance and or TC matching available on request • Material categorization: for definitions of compliance please see www.vishay....
Controlled temperature coefficient Excellent high frequency characteristics Exceptionally low noise; typically 0.10 μV/V Low voltage coefficient to ± 5 ppm/V Special tolerance and or TC matching available on request • Material categorization: for definitions of compliance please see www.vishay....