Polymerase Fidelity: What is it, and what does it mean for your PCR?质控、安全 & 法规 质控分析 每一新批次的 NEB 产品都要经过质控,以满足指定的质量标准,对特定产品的质量标准和每一批次的检测数据可以通过产品说明表格、COA、产品信息卡或者产品手册进行查阅或下载。关于 NEB 产品质控的详细信息,可从此处...
Why Choose Q5® High-fidelity Polymerase? 5 Tips for Setting Up Your PCR Choose the Right DNA Polymerase for PCR Behind the paper: Examining Sources of Error in PCR by Single-Molecule Sequencing Overview of PCR Why is Tm Important in Primer Design? Why Choose Q5® High-fidelity...
Does Q5®High-Fidelity DNA Polymerase exhibit a strand displacement activity? What are the properties of this polymerase (fidelity, product ends, max amplicon, modified base incorporation, etc.)? How should I determine the appropriate annealing temperature for my reaction? What should the final pri...
NEBNext® Q5U® Master Mix contains a modified version of Q5® High Fidelity DNA Polymerase, optimized for amplification of uracil-containing NGS libraries. This hot start formulation is designed to read through uracils, providing robust, high-fidelity and uniform library amplification. NEB...
咨询热线:4006-400-850 QQ2885367975Shelf Life(month):12 产地:USA 存储条件:+4C Q5 热启动超保真DNA聚合酶延伸速度 6kb/min;扩增长度 ≤ 20kb;高保真度: 100X Taq;1 Unit/50ul反应体系;平末端。Q5 无与伦比的扩增保真性( Taq 酶的100倍)及其超低的错配率,建立了
Can I use Q5U Hot Start High-Fidelity DNA Polymerase to amplify FFPE DNA? What ends will my PCR products have? What are Hot Start and WarmStart®polymerases and when would I use them? How should I determine the appropriate annealing temperature for my reaction?
Q5High-FidelityONAPolymeraseD^5ul05Hl002U仙 5X06HighGCEnhancerOptional) (WD (10Ml)g Njclease-FrecWalerto25plto50m Notes:Gentlymixthereaction.Collectallliquidtothebottomofthetubebyaquickspinifnecessary.Overlaythesamplewith mineraloilifusingaPCRmachinewithoutaheatedlid.TransferPCRtubestoaPCRmachineandbegin...
6、enerated with Q5 High-Fidelity DNA Polymerase. When used at a final concentration of 1X, the Q5 Reaction Buffer provides the optimal Mg+concentration. Amplification of some difficult targets, like GC-rich sequences, may be improved by the addition of 1X Q5 High GC Enhancer. The Q5 High...
英文名称 Q5® High-Fidelity DNA Polymerase 储存 -20°C Concentration 2 X 产地 美国 售卖区域 全国 质检 通过质检 发货地 北京 服务 提供售前售后服务 了解聚合酶详情 北京百奥创新科技有限公司 店铺信息 成立时间 2017-02-16 注册资本 100万元 主营商品: 试剂;耗材;仪器;转染试剂;实验耗材 ...
https://www.neb.com/protocols/2012/09/27/pcr-using-q5-high-fidelity-dna-polymerase-m0491 Protocol 1.Please note that protocols with Q5® High-Fidelity DNA Polymerase may differ from protocols with other polymerases. Conditions recommended below should be usedfor optimal performance. Reaction Setup...