1.如果要加在序列的 5’端,就在酶切位点识别碱基序列红色的 5’端加上相应的碱基黑色,相同如果要在 3’端加保护碱基,就在酶切位点识别碱基序列红色的3’端加上相应的碱基黑色;2.切割率:正确识别并酶切的效率3;加保护碱基时最好选用切割率高时加的相应碱基;...
AgeI-HF R3552L 1,500 units ￥3,079 ￥2,309 R3552S 300 units ¥709 ￥532 AhdI R0584L 5,000 units ￥3,339 ￥2,504 R0584S 1,000 units ￥739 ￥554 R0584V 500 units ¥389 ￥292 AluI R0137L 5,000 units ￥2,789 ￥2,092 R0137S 1,000 units ¥669 ￥502 R0...
PCR设计引物时酶切位点的保护 切割率% 酶寡核苷酸序列 2 hr20 hr Acc I G GTCGAC C CG GTCGAC CG CCG GTCGAC CGG 0 Afl III C ACATGT G CC ACATGT GG CCC ACATGT GGG >90 >90 >90 >90 Asc I GGCGCGCC A GGCGCGCC T TT GGCGCGCC AA >90 >90 >90 >90 >90 >90 Ava I C CCC...
Pac19842II x CCTTGA PacIII x GTAATC PaeI GCATG/C SphI§ R0182 GCATG/C SphI, SphI-HF SphI-HF R3182 GCATG/C Pae8506I x CATCGAR Pae10662III x TGACGAG PaePA99III x AAGAYC PaeR7I C/TCGAG PaeR7I R0177 C/TCGAG Sfr274I, SlaI, XhoI XhoI R0146 C/TCGAG PagI T/CAT...
NCG281 Pac Man NebuaNebulaEnlargeDescription Rob Gendler has provided an excellent write-up on NGC281. Click here. In summary, NGC281 is a bright, very young HII emission region in the Perseus Arm of our galaxy whose energizing source is the young cluster of OB stars, IC1590, that is ...
pcr设计引物时酶切位点的保护 酶 募核甘酸序列 切割率 2 hr 20 hr acc i ggtcgac c o 0 cggtcgac cg 0 0 ccg gtcgac cgg 0 0 afl iii cacatgt g 0 0 cc
Narrowband Pacman Nebtelecasterguru
Hi @benjjneb I'm Hanbeen Kim. I have raw data set sequenced by PacBio technology. I have two significant issues when processing the denoising command using dada2. First, when I'm trying to run dada2 in qiime2 using the below command; qii...
多重耐药革兰阴性杆菌血流感染课件 starter_unit2-1课件(1) 第二节 城市化与城市环境问题 南方团队合作培训 (2) 例1计算在电偶极子延长线上任一点A的场强 会计学原理习题 主管培训-如何面对自我成长的挑战 临时心脏起搏器植入术前准备 工程预算及管理 小学音乐常规教学-手号...
(CGAT/CG) Dpn II, Pvu I Pac I Mse I Pvu I Dpn II, Pvu I Ava I (C/CCGGG) Age I, BsaW I, BspE I, BsrF I (R/CCGGY), NgoM IV, SgrA I (CR/CCGGYG) Hpa II, Nci I, ScrF I (C/TCGAG) Xho I Ava I, Taq I, Xho I (C/TCGAG) Sal I Taq I (C/CCGGG) Xma ...