NEBNext Ultra II 可在 PCR-free 实验流程中实现卓越的产量 以100 ng 人 NA19240 基因组 DNA 为样本制备文库,文库制备试剂盒如图所示,文库制备按照生产商推荐方法进行,不进行扩增步骤。使用 NEBNext 文库定量试剂盒(Illumina)通过 qPCR 测定文库产量。NEBNext Ultra II 文库制备试剂盒的产量最高。 NEBNext Ultra...
Where can I find protocols for using the NEBNext Ultra II FS DNA PCR-free Library Prep Kit for Illumina? Where can I find protocols for using NEBNext®DNA Library Prep reagents? 说明书 产品说明书包含产品使用的详细信息、产品配方和质控分析。
I don’t know of any other NGS library prep kit that works so well with small genomes/DNA fragments e.g. bacterial, phage genomes, plasmids, PCR products. –Anton Bryksin, Ph.D., Director, Molecular Evolution Core, Parker H. Petit Institute for Bioengineering and Bioscience One or more ...
In today’s high-demand genomics landscape, researchers need library prep solutions that can keep pace. At the University of Michigan’s Advanced Genomics Core, Director Dr. Olivia Koues shares how her team streamlined operations, improved turnaround times, and handled diverse, challenging samples ...
need library prep solutions that can keep pace. At the University of Michigan’s Advanced Genomics Core, Director Dr. Olivia Koues shares how her team streamlined operations, improved turnaround times, and handled diverse, challenging samples using the NEBNext UltraExpress® Library Prep Kits. ...
What is the main application of the NEBNext Ultra II FS DNA PCR-free Library Prep Kit? What additional reagents do I need to complete the Ultra II FS DNA PCR-free workflow? Why is DTT included in the Ultra II FS DNA PCR-free kit?
Which NEBNext Oligos can be used with this library prep kit? Can I use this NEBNext kit with adaptors and primers from other vendors than NEB? What do I do if I see a precipitate in the Ultra II End Prep Reaction Buffer? 问题解决指南 ...
The NEBNext Ultra II FS DNA PCR-free Library Prep Kit contains the enzymes and buffers required to convert a broad range of intact DNA input amounts into libraries for next-generation sequencing on the Illumina platform, all without an amplification step. The fast, user-friendly workflow include...
In this issue, discover faster NGS library prep, improved EM-seq kits for methylation analysis, viral nucleic acid extraction from milk, PCR and DNA cleanup tips, enzyme innovations, and wild yeast's role in brewing flavorful beer. Learn About NEB's Research ...
Each component in the NEBNext Ultra II DNA Library Prep Kit from NEB has been reformulated, resulting in a several-fold increase in library yield with as little as 500 picograms of input DNA. These advances deliver unprecedented performance, while enabling lower inputs and fewer PCR cycles. Ge...