KLD Enzyme Mix Reaction Protocol (M0554) 工具& 资源 Web 工具 NEBaseChanger FAQs & 问题解决指南 FAQs Is the KLD Mix (NEB#M0554) the same product as the component of the Q5 Site-Directed Mutagenesis Kits (E0554S and E0552S)? What polymerases can be used to amplify the fragments that wi...
Use KLD Enzyme Mix (NEB #M0554) for kinase, ligase and DpnI enzymatic activities in single mix Reduce screening of correct mutants with extremely accurate and robust Q5 High-Fidelity DNA Polymerase Take advantage of automation compatible room temperature reaction set up using a Hot Start ...
What plasmid sizes can be amplified using the Q5® Site-Directed Mutagenesis Kit? Do I need to purify my plasmid before or after the KLD reaction when using the Q5® Site-Directed Mutagenesis Kit? How do I design primers to use with the Q5® Site-Directed Mutagenesis Kit? What should...
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The use of a master mix, a unique multi-enzyme KLD enzyme mix, and a fast polymerase ensures that, for mostplasmids, the mutagenesis reaction is complete in less than two hours. Figure 2: Q5 Site-Directed Mutagenesis Overview. This kit is designed for rapid and efficient incorporation of in...
PCR Product 1 µl 2X KLD Reaction Buffer 5 µl 1X 10X KLD Enzyme Mix 1 µl 1X Nuclease-free Water 3 µl Incubate for 5 minutes at room temperature. Step 3: Transformation 1. Add 5 µl of KLD mix to 50 µl of chemically-competent cells. 2. Incubate on ice for 30 ...