Assembly enables virtually error-free joining of DNA fragments, even those with 5´- and 3´-end mismatches. This flexible mix enables simple and fast seamless cloning utilizing a proprietary high-fidelity polymerase. Find out why NEBuilder HiFi is the next generation of DNA assembly and ...
产品名称 高保真 DNA 组装预混液 产品规格 50 reactions 型号 E2621L 用途范围 用于科研 是否进口 是 加工定制 否 英文名称 HiFi DNA Assembly Master Mix 储存 -20°C Concentration 2 X 产地 美国 质检 通过质检 售卖区域 全国 服务 提供售前售后服务 发货地 北京 品牌 NEB 价格说明 价格:商...
NEB HiFI DNA组装试剂盒就是利用同源重组的方法。没有菌落长出来，没菌可挑呀 发自小木虫Android客户端...
During assembly, the T5 exonuclease activity chews back the 5-prime end of the DNA fragment, generating a 3-prime single-stranded overhang, which allows the oligo to anneal with it. The high-fidelity DNA polymerase activity fills the gap, and DNA ligase seals the nicks. The T5 ...
使用说明 常见问题 NEBuilder HiFi DNA Assembly Master Mix was developed to improve the efficiency and accuracy of DNA assembly. This method allows for seamless assembly of multiple DNA fragments, regardless of fragment length or end compatibility. This method has been used to assemble either single...
coli 感受态细胞是广泛使用的 DH5α 的衍生菌株。它具有 T1 噬菌体抗性且属于 endA 缺陷型,可用于高质量质粒的制备。 高转染效率菌株,是多种应用的理想选择 有多种规格可供选购,包括一次性管、200 μL 管、96 和 384 孔板和 12 x 8 联管 不另外收取感受态细胞运输的干冰附加费 随附Outgrowth 培养基 无...
What type of competent cells are suitable for transformation of DNA constructs created using NEBuilder HiFi DNA Assembly Master Mix? How should I store SOC Outgrowth Medium? The SOC I received with my competent cells recommends storage at either room temperature or 4°C, however, when I purchase...
操作说明、说明书 & 用法 操作说明 In vitrodigestion of DNA with Cas9 Nuclease,S. pyogenes(M0386) Using recombinant Cas9 nuclease to assess locus modification in genome editing experiments (#M0386) 应用实例 Bridging dsDNA with a ssDNA Oligo and NEBuilder® HiFi DNA Assembly to create an sgRNA-...
Supplemental Protocol 1: Generation of DNA fragments by PCR assembly of pooled oligos (NEB #M0689) Supplemental Protocol 2: Using colony PCR to identify positive clones (NEB #M0689) 说明书 产品说明书包含产品使用的详细信息、产品配方和质控分析。 manualM0689工具...
Closer to the clinic: Optimized DNA assembly for personalized medicine research In this webinar, we will discuss how data-optimized Golden Gate Assembly and NEBuilder® HiFi DNA Assembly mixes and protocols can be used to enable new and massively parallel approaches to personalized medicine. ...