高保真 DNA 大片段组装试剂盒 NEBuilder HiFi DNA Assembly Master Mix has been reformulated with components containing Recombinant Albumin (rAlbumin) beginning with Lot #10238675. 对于大于 15 kb 组装片段的转化,推荐使用 NEB 10-beta E
操作说明、说明书 & 用法 操作说明 In vitrodigestion of DNA with Cas9 Nuclease,S. pyogenes(M0386) Using recombinant Cas9 nuclease to assess locus modification in genome editing experiments (#M0386) 应用实例 Bridging dsDNA with a ssDNA Oligo and NEBuilder® HiFi DNA Assembly to create an sgRNA-...
What type of competent cells are suitable for transformation of DNA constructs created using NEBuilder HiFi DNA Assembly Master Mix? How should I store SOC Outgrowth Medium? The SOC I received with my competent cells recommends storage at either room temperature or 4°C, however, when I purchase...
产品名称 高保真 DNA 组装预混液 产品规格 50 reactions 型号 E2621L 用途范围 用于科研 是否进口 是 加工定制 否 英文名称 HiFi DNA Assembly Master Mix 储存 -20°C Concentration 2 X 产地 美国 质检 通过质检 售卖区域 全国 服务 提供售前售后服务 发货地 北京 品牌 NEB 价格说明 价格:商...
Protocol for assembling annealed DNA oligonucleotides and a double-stranded DNA vector using NEBuilder HiFi DNA Assembly (NEB #E2621) 说明书 产品说明书包含产品使用的详细信息、产品配方和质控分析。 manualE2621_E5520 使用指南 Guidelines for using NEBuilder®HiFi DNA Assembly ...
说明书 产品说明书包含产品使用的详细信息、产品配方和质控分析。 manualE0554 应用实例 Improved methods for site-directed mutagenesis using NEBuilder®HiFi DNA Assembly Master Mix 工具& 资源 Web 工具 NEBaseChanger FAQs & 问题解决指南 FAQs How do I design primers to use with the Q5® Site-Directe...
产品名称: 高保真 DNA 组装预混液 产品规格: 50 reactions 用途范围: 用于科研 是否进口: 是 加工定制: 否 英文名称: HiFi DNA Assembly Master Mix 储存: -20°C Concentration: 2 X 产地: 美国 质检: 通过质检 售卖区域: 全国 服务: 提供售前售后服务 发货地: 北京 价格说明 价格:商...
NEBUILDER HIFI DNA ASSEMBLY® Removal of 3´ end Mismatches Fast transformation plating with glass beads Should I choose chemically competent or electrocompetent cells? Differences between electrocompetent and chemically competent cells NEBUILDER HIFI DNA ASSEMBLY® Removal of 3´ end Mismatches...
NEBuilder Assembly Tool can be used to design primers for NEBuilder HiFi DNA Assembly or Gibson Assembly reactions.
NEBuilder HiFi DNA Assembly Master Mix was developed to improve the efficiency and accuracy of DNA assembly. This method allows for seamless assembly of multiple DNA fragments, regardless of fragment length or end compatibility. This method has been used to assemble either single-stranded oligonucleotid...