Breaking through the Limitations of Golden Gate Assembly Efficient Adaptor Ligation for the Preparation of dsDNA Libraries using the Blunt/TA Ligase Master Mix Generating Oligonucleotide Probes using Immobilized T4 DNA Ligase minimizes sample loss and saves time...
Protocols for High-throughput cloning and automation solutions Golden Gate Assembly Protocol using PaqCI® (NEB #R0745) and NEBridge Ligase Master Mix (NEB #M1100)Videos 1 of 10 Introduction to the NEBExpress® Cell-free Protein Synthesis System Watch this tutorial explaining the st...
Golden Gate Assembly Protocol for using NEBridge®Golden Gate Assembly Kit (BsmBI-v2) (NEB #E1602) Transformation Protocol for NEBridge®Golden Gate Assembly Kit (BsmBI-v2) (NEB #E1602) Recommended Screening Protocols for NEBridge®Golden Gate Assembly Kit (BsmBI-v2) (NEB #E1602) 35-...
Learn about an accessible method for shuffling DNA using NEBridge® Golden Gate Assembly to recombine proteins and find new properties. Application Note Read about how NEB scientists extract and detect viral nucleic acids from milk and subsequently amplify them using RT-qPCR or RT-LAMP. Web ...
除此之外,我们还盘点了NEB众多明星产品加入至本次促销,“星选”内切酶、金牌T4连接酶、超高保真Q5聚合酶等全部网罗其中。 1. 限制性内切7.5折 高品质内切酶,就选 NEB!注:V包装不参与活动
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NEB has developed convenient kits (using BsmBI-v2 and BsaI-HFv2) for performing Golden Gate Assembly. For additional flexibility and convenience for users, NEBridge® Ligase Master Mix performs high efficiency and high-fidelity Golden Gate Assembly with a broad assortment of NEB Type IIS restrict...
NEB® Gibson Assembly Master Mix & Gibson Assembly Cloning Kit Successful assembly of multiple DNA fragments, regardless of fragment length or end compatibility NEBridge Golden Gate Assembly Kit (BsmBI-v2) Efficient, ordered assembly of multiple fragments in a single reaction DIGESTIONHigh...
Learn about an accessible method for shuffling DNA using NEBridge® Golden Gate Assembly to recombine proteins and find new properties. Application Note Read about how NEB scientists extract and detect viral nucleic acids from milk and subsequently amplify them using RT-qPCR or RT-LAMP. ...
Blunt Enzyme Mix 1 µl Nuclease-free Water To 25 µl Incubation 15 minutes for RE-digested DNA/sheared or 30 minutes for nebulized DNA or PCR products Heat Inactivation 70°C for 10 minutes * PCR-generated DNA must be purified before blunting using a commercial purification kit (NEB #T...