要合成高比活性的放射性 RNA 探针或具有可完全替代一个或多个经修饰的核苷酸的 RNA,建议使用 T7 高效 RNA 合成试剂盒(NEB #E2040),其中单独提供四种核苷酸。 DNA 模板制备: 使用HiScribe T7 快速高效 RNA 合成试剂盒进行体外转录时,可使用线性化质粒 DNA、PCR 产物或合成 DNA 寡核苷酸作为模板,转录的序列上游...
HiScribe™ T7 High Yield RNA Synthesis Kit E2040S 50 rxns HiScribe™ T7 Quick High Yield RNA Synthesis Kit E2050S 50 rxns HiScribe™ T7 ARCA mRNA Kit (with tailing) E2060S 20 rxns HiScribe™ T7 ARCA mRNA Kit E2065S 20 rxns T7 RNA Polymerase M0251L 25,000 units T7 RNA Po...
HiScribe T7 High Yield RNA Synthesis Kit E2040S 50 rxns ￥2,349 ¥1,762 HiScribe T7 Quick High Yield RNA Synthesis Kit E2050S 50 rxns ¥2,999 ¥2,249 HiScribe T7 ARCA mRNA Kit (with Tailing) E2060S 20 rxns ¥4,199 ¥3,149 HiScribe T7 ARCA mRNA Kit E2065S 20 ...
HiScribe™ T7 High Yield RNA Synthesis Kit E2040S 50 rxns ¥2,449 ¥1,714 HiScribe™ T7 Quick High Yield RNA Synthesis Kit E2050S 50 rxns ¥3,059 ¥2,141 HiScribe™ T7 ARCA mRNA Kit (with tailing) E2060S 20 rxns ¥4,399 ¥3,079 HiScribe™ T7 ARCA mRNA ...
e2040-hiscribe-t7-high-yield-rna-synthesis-kit SP6 RNA 聚合酶 m0251-t7-rna-polymerase rNTP 混合液 小鼠RNase 抑制剂 m0307-rnase-inhibitor-human-placenta 注意事项 NEB PCR 克隆试剂盒包含的试剂足以进行 20 次 10 μl 的克隆反应。试剂盒还提供引物,便于进行下游菌落 PCR 筛选和/或测序。
R3131S NheI-HF 1000units 849 neb 1周 上海起发 E2040S HiScribe T7 High Yield RNA Synthesis Kit - 50 reactions 50rxns 2759 neb 1周 上海起发 R3193S NcoI-HF 1000units 789 neb 1周 上海起发 R3133S SpeI-HF 500units 849 neb 1周 上海起发 R3539L BbsI-HF 1500units 3869 neb 1周 上海...
followed by purification using a spin column (e.g., Monarch® PCR & DNA Cleanup Kit (5 μg) NEB #T1030) or phenol extraction/ethanol precipitation. Although linearization of plasmid involves multiple steps, the process is easier to scale for the generation of large amounts of template for...
NEBNext®Small RNA 建库流程经过创新性优化,在保证制备高产量和多样性文库的同时,可以最大限度减少接头二聚体的形成。NEBNext® Small RNA 文库制备试剂盒 1 包含接头和具有 12 种 Index 的多样本引物,可实现多样本建库。试剂盒 NEB #E7580 和 NEB #E7560 中包含用于多样本建库的、不同的 Index 引物。
NEB#E2050S 50reactions NaNodrop ™ ist em k fThe m Fishe Scientific. SYBr ® is egiste e t em k fLifeTechn l gies,Inc. ISO9001 Registered Quality Management ISO14001 Registered Environmental Management ISO13485 Registered MedicalDevices ...
OneTaq® RT-PCR Kit E5310S 30 rxns ￥1,259 ￥944 HiScribe™ T7 High Yield RNA Synthesis Kit E2040S 50 rxns ￥2,349 ￥1,762 HiScribe™ T7 Quick High Yield RNA Synthesis Kit E2050S 50 rxns ￥2,999 ￥2,249 HiScribe™ T7 ARCA mRNA Kit (with Tailing) E2060S 20 ...