DNA Ligation ds: Mass ⇄ Moles ds: Mass → Ends ss: Mass ⇄ Moles RNA ss: Mass ⇄ Moles Protein Mass ⇄ Moles General OD260 Dilution Molarity Genome Editing sgRNA Designer qPCR NGS Library Quant General qPCRLigation CalculatorThis tool will calculate the mass of insert required at ...
NEBioCalculator® Use this tool for your scientific calculations and conversions for DNA and RNA. Options include conversion of mass to moles, ligation amounts, conversion of OD to concentration, dilution and molarity. Additional features include sgRNA Template Oligo Design and qPCR library quantifica...
DNA:用于连接的纯化 DNA 可溶于 dH2O(推荐使用 Milli-Q™ 水或同等纯度的水);也可使用 TE 或其它稀释缓冲液。为获得最佳连接效果,在添加 2X 快速连接缓冲液之前,DNA 和插入片段的体积应为 10 μl。若 DNA 体积大于 10 μl,则相应增加 2X 快速连接缓冲液的体积,使其体积为反应体系的 50%,并相应地增加...
T4 多聚核苷酸激酶能够催化 ATP 的γ-位磷酸基团转移到寡核苷酸链(双链或单链 DNA 或 RNA)的 5´-羟基末端以及 3´-单磷酸核苷上。T4 多聚核苷酸激酶还具有 3´ 磷酸酶活性,将 3´-磷酸基团从寡核苷酸的 3´ 磷酸末端、脱氧 3´-单磷酸核苷和脱氧 3´-二磷酸核苷上水解掉(1)。 产品来源...
Use Tm Calculator to calculate optimum annealing temperature for PCR primers when using NEB polymerases and buffers. Use NEBioCalculator to convert DNA or RNA mass to moles, calculate insert ligation amounts, convert OD to concentration, and calculate basic dilution and molarity. NEB Tools for ...
T4 多聚核苷酸激酶能够催化 ATP 的γ-位磷酸基团转移到寡核苷酸链(双链或单链 DNA 或 RNA)的 5´-羟基末端以及 3´-单磷酸核苷上。T4 多聚核苷酸激酶还具有 3´ 磷酸酶活性,将 3´-磷酸基团从寡核苷酸的 3´ 磷酸末端、脱氧 3´-单磷酸核苷和脱氧 3´-二磷酸核苷上水解掉(1)。
Use Tm Calculator to calculate optimum annealing temperature for PCR primers when using NEB polymerases and buffers. Use NEBioCalculator to convert DNA or RNA mass to moles, calculate insert ligation amounts, convert OD to concentration, and calculate basic dilution and molarity. NEB Tools for ...
Use Tm Calculator to calculate optimum annealing temperature for PCR primers when using NEB polymerases and buffers. Use NEBioCalculator to convert DNA or RNA mass to moles, calculate insert ligation amounts, convert OD to concentration, and calculate basic dilution and molarity. NEB Tools for ...
Calculator to calculate optimum annealing temperature for PCR primers when using NEB polymerases and buffers. Use NEBioCalculator to convert DNA or RNA mass to moles, calculate insert ligation amounts, convert OD to concentration, and calculate basic dilution and molarity. NEB Tools for iOS also ...
in the proper orientation. (3) The NEBiocalculator® Tool (nebiocalculator.neb.com) can be used for molarity calculations. (4) Can be increased to 25 μl volume if required due to DNA component volumes; add additional 0.5 μl T4 DNA Ligase Buffer (10X). Reaction...