细胞复苏培养基对转化效率的影响:按照所提供的高效级转化操作流程,用 100 pg pUC19 对照 DNA 转化 50 μl NEB 5-alpha E.coli 感受态细胞,不同反应之间使用的复苏培养基不同。NEB SOC Outgrowth 培养基的转化效率最高。从NEB 5-alpha 的高转化效率中获益:比较NEB 5-alpha 与 DH5α 的转化效率,按照生产...
NEB 5-alphaE. coli感受态细胞是广泛使用的 DH5α 的衍生菌株。它具有 T1 噬菌体抗性且属于endA缺陷型,可用于高质量质粒的制备。 非常适用于亚克隆效级转化,如质粒转化或常规亚克隆 不另外收取感受态细胞运输的干冰附加费 无动物来源产品 高效级版本同样在售 ...
NEB 5-alpha Electrocompetent Competent E. coli is a derivative of the popular DH5α. It is T1 phage resistant and endA deficient for high-quality plasmid preparations. Optimized for high efficiency transformation by electroporation No dry ice surcharge on competent cell shipments Outgrowth medium ...
NEB 5-alpha (High Efficiency) Versatile cloning strain DH5α™ derivative 1-3 x 109 (8) n/a 50, 200, 96, 384, Strips • K12 • • • • none Dam+, Dcm+,M. EcoKI+ NEB 5-alpha F´ Iq (High Efficiency) Toxic gene cloning F´ strain with extremely high transformat...
NEB 5-alpha ElectrocompetentE. colicells are optimized for high transformation efficiency by electroporation. These cells are ideal for DNA library constructions and all cloning purposes. 重点 DH5α™ derivative Efficient transformation of unmethylated DNA derived from PCR, cDNA and many other sources...
分别将 pUC19 转化进 NEB Turbo E.coli、Invitrogen Mach1 和 DH5α 感受态细胞。将过夜培养的菌落(菌落大小:NEB Turbo 直径约 2.4 mm,Mach1 直径约 1.8 mm,DH5α 直径约 1.2 mm)接种于 25 ml 试管中的 5ml LB/Amp 中,在 37℃ 条件下以 250 rpm 转速摇动细胞。生长 4 小时后,测量细胞的 O.D.600...
such as plasmid transformation or routine subcloning(e.g,. inserting a 1 kb fragment into a 2 kb vector). The efficiency ofthese cells is not sufficiently high for most other cloningapplications. For that, we recommend NEB® 5-alpha CompetentE. coli(High Efficiency) (NEB #C2987) which ...
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该试剂盒用 Q5 热启动超保真 DNA 聚合酶进行 PCR 扩增,PCR 产物直接加入到酶混合液中 [Kinase-Ligase-DpnI (KLD)],进行环化和模板去除反应(5 分钟)(见图 1),然后转入高效级 DH5-alpha E. coli 感受态细胞(本试剂盒不含感受态细胞,需客户自备) 特性 ● 对于各种模板的突变均能稳定,高效 的进行指数扩增 ...
Product Name:NEB 10-BETA electro-competent cells Synonyms: CAS: MF: MW:0 EINECS: Mol File:Mol File Browse by Nationality Suppliers >Global suppliers Beijing (1) Shanghai (3) Member (4) All (4) EnquiryPlease select the suppliers Recommend You Select Member Companies Company Name: Shang...