Golden Gate Assembly Protocol for using NEBridge®Golden Gate Assembly Kit (BsmBI-v2) (NEB #E1602) Transformation Protocol for NEBridge®Golden Gate Assembly Kit (BsmBI-v2) (NEB #E1602) Recommended Screening Protocols for NEBridge®Golden Gate Assembly Kit (BsmBI-v2) (NEB #E1602) 35-...
BsmBI-v2(R0739) 是一款 IIS 型限制性内切酶,其为 Esp3I 的同裂酶。BsmBI-v2 为 BsmBI(R0580)的升级替代产品,该酶被改造以在Golden Gate组装(无缝克隆)实验中具有更佳性能。NEB 使用 “v2”或“HF”来命名人工改造过的酶。 特性 省时酶,5-15分钟完成酶切 应用于 Golden Gate 组装 IIS 型内切酶,识别...
NEB Golden Gate组装试剂盒(BsmBI-v2)是BsmBI-v2和T4 DNA连接酶的优化预混液。BsmBI-v2是NEB公司研发的BsmBI的升级版本,在Golden Gate组装中的表现优于原酶。这些酶联合使用可完成多个插入片段/模块的组装,也可用于单插入片段克隆/文库构建。试剂盒提供pGGAselect目的质粒,作为组装骨架。该多功能目的质粒上定向克...
BsmBI-v2是NEB公司研发的BsmBI的升级版本,在Golden Gate组装中的表现优于原酶。这些酶联合使用可完成多个插入片段/模块的组装,也可用于单插入片段克隆/文库构建。试剂盒提供pGGAselect目的质粒,作为组装骨架。该多功能目的质粒上定向克隆有BsmBI-、BsaI-和BbsI-的识别位点,使其能方便地与这三种在Golden Gate中最常...
BsmBI-v2 Esp3I PaqCI SapI BspQI操作说明、说明书 & 用法 操作说明 Golden Gate Assembly Protocol for NEBridge® Ligase Master Mix (NEB #M1100) Golden Gate Assembly Protocol using PaqCI® (NEB #R0745) and NEBridge Ligase Master Mix (NEB #M1100) 使用指南 NEBridge® Ligase Master Mix Pro...
Golden Gate Assembly of 35 fragments using BsmBI- v2 限制酶与 T4 DNA连接酶,随后转化为 NEB 10-beta CompetentE。大肠杆菌(高效)。 材料 NEBridge® Golden Gate Assembly Kit (BsmBI-v2) (NEB #E1602)pGGAselect Destination Plasmid*NEB 10-beta CompetentE.大肠杆菌(NEB #C3019)NEB 10-beta/稳定...
NEBuilder® 高保真 DNA 组装预混液 NEBridge® 连接酶预混液 NEBridge® Golden Gate 组装试剂盒(BsmBI-v2) Related Content Interview with Kate Creasey Krainer: Enabling Global Agricultural Growth Monarch® Nucleic Acid Purification Kits High-throughput cloning and automation solutions获取...
NEBuilder® 高保真 DNA 组装预混液 NEBridge® 连接酶预混液 NEBridge® Golden Gate 组装试剂盒(BsmBI-v2) Related Content Interview with Kate Creasey Krainer: Enabling Global Agricultural Growth Monarch® Nucleic Acid Purification Kits High-throughput cloning and automation solutions获取...
BsmBI-v2限制性内切酶 R0739S 200 units PsiI-v2限制性内切酶 R0744L 2,000 units PsiI-v2限制性内切酶 R0744S 400 units PaqCI限制性内切酶 R0745L 1000 units PaqCI限制性内切酶 R0745S 200 units EcoRI-HF限制性内切酶 R3101L 50,000 units ...
Learn how to push the limits of your Golden Gate Assembly, and try one of our convenient kits (using BsmBI-v2 or BsaI-HFv2) The efficient and seamless assembly of DNA fragments, commonly referred to as Golden Gate Assembly (1,2), has its origins in 1996, when for the first time it...