33、1006537ASapINEBuffer 4755001006537CSau3AIE3NEBuffer 1+ BSA10050101006537ASau96IE3NEBuffer 4501001001008037ASbfIRBCNEBuffer 4755001006537ASbfI-HF ?NEBuffer 4502501006537BScaINEBuffer 3NRNR100 ddNR8037AScaI-HF ?NEBuffer 4100100101006537BScrFI口NEBuffer 41001001001006537ASexAINEBuffer 4 + BSA10075501006 34...
Diluent BaeGI NEBuffer 1 100 75 10 50 65 37 A BaeI NEBuffer 4 + BSA + SAM 50 100 50 100 65 25 A BamHI NEBuffer 3 + BSA 75 100 100 dd 100 No 37 A BamHI-HF™ NE 10、Buffer 4 100 50 10 100 No 37 A BanI NEBuffer 4 + BSA 50 100 50 100 65 37 A BanII NEBuffer ...
| Double Digestion | Heat Inactivation | Activity at 37°C | Diluent Buffers | Time-Saver Enzymes | High Fidelity (HF) Restriction Enzymes This chart has been updated to reflect the change of 54 restriction enzymes to NEBuffer 4 on Nov.1, 2008. Click for the Chart Legend, Icon ...
在本表中没有列出的酶,则通常需在识别位点两端至少加上6个保护碱基,以确保酶切反应的进行 酶 寡核苷酸序列 切割率% 2 hr 20 hr Acc I GGTCGACC CGGTCGACCG CCGGTCGACCGG 0 0 0 0 0 0 Afl III CACATGTG CCACATGTGG CC...
yKyBQpl1xXX18WHWzpOezdNC-4JoAWl9vv09XFydWwzNb8X-7yvmURGiRqilmqKSaryz3H2V6AnS8gv9hmHyErWfAXfzWvPW7c-KV27ffGy2vm6Ysxyz409n-Yk0W5kL7J5o1ytndTTkfHd650N4NjUTaKmypF93Gy_XRBPAZTTGpt8mztwRsqk_pfB_PL8Lu9aOgwq3a9meFVBcQ4m2nwLZrbFzcbN9-aj283Jmasc9fN-TP_RvezO4vKZJpZdJ63xkrt-afG6MVI...
1.The increased specificity for the BamHI-HF™ cut site has increased binding of the enzyme to the DNA and the enzyme may remain attached to the DNA during gel electrophoresis. 2.Star activity may result from a glycerol concentration of >5%本...
32、6537asali-hfnebuffer 4101001001006537asapinebuffer 4755001006537csau3ainebuffer 1 + bsa10050101006537asau96inebuffer 4501001001008037asbfinebuffer 4755001006537asbfi-hfnebuffer 4502501006537bscainebuffer 3nrnr100 ddnr8037ascai-hfnebuffer 4100100101006537bscrfinebuffer 41001001001006537asexainebuffer 33、 4 ...
1.The increased specificity for the BamHI-HF™ cut site has increased binding of the enzyme to the DNA and the enzyme may remain attached to the DNA during gel electrophoresis. 2.Star activity may result from a glycerol concentration of >5%本...
32、101001001006537ASapINEBuffer 4755001006537CSau3AINEBuffer 1 + BSA10050101006537ASau96INEBuffer 4501001001008037ASbfINEBuffer 4755001006537ASbfI-HFNEBuffer 4502501006537BScaINEBuffer 3NRNR100 ddNR8037AScaI-HFNEBuffer 4100100101006537BScrFINEBuffer 41001001001006537ASexAINEBuffer 4 + BSA10075501006537 33、ASfaNI...
33、1001001006537ASapINEBuffer 4755001006537CSau3AIE3NEBuffer 1 + BSA10050101006537ASau96IE3NEBuffer 4501001001008037ASbfIRBCNEBuffer 4755001006537ASbfI-HF ?NEBuffer 4502501006537BScaINEBuffer 3NRNRdd100NR8037AScaI-HF?NEBuffer 4100100101006537BScrFI口NEBuffer 41001001001006537ASexAIE3NEBuffer 4 + BSA1007 34...