NEB 限制性内切酶双酶切步骤Nebiolabs-China 立即播放 打开App,流畅又高清100+个相关视频 更多 954 1 01:45 App 用NEBcloner 进行双酶切 319 0 01:05 App 限制性内切酶未切割 DNA 的原因 1002 0 00:54 App 限制性内切酶酶切操作:DNA 的进末端切割 296 0 00:54 App 限制性酶切的省时操作步骤 445 0...
NEB--推荐双酶切系统(最新版-修订).pdf,Select 1st Select 2nd GO enzyme: enzyme: % Activity in Enzyme Cat# Temp Supplied Supplements NEBuffer NEBuffer BSA SAM 1 2 3 4 BamHI* R0136 37°C NEBuffer 3 Yes No 75 100 100 100 KpnI* R0142 37°C NEBuffer 1 Yes No
1、Select1stenzyme:Select2ndGOenzyme:EnzymeCat#TempSuppliedNEBufferSupplements%ActivityinNEBufferBSASAM1DoubleDigestRecommendation(s)forBamHI+Kpnl:Doubledigestisnotrecommended.Asequentialdigestisrequired,usingeachenzymeinitssuppliedNEBuffer.Note:*BamHIhasaHighFidelityversionBamHI-HF(R3136)KpnlhasaHighF 2、...
onbufferpagesandonthedatacardsentwitheachenzyme.TheActivityChartforRestrictionEnzymesratesthepercentageactivityofeachrestrictionendonucleaseinthefourstandardNEBuffersandNEBufferEcoRI.PleaseuseNEBDoubleDigestFindertoperformadoubledigestcalculation.SuggestedNEBuffersforDoubleDigestionEnzymeAatIIAvrIIBamHIBglIIBsgIEagIEcoRIEcoRV...
NEB--推荐双酶切系统(最新版-修订) 热度: neb内切酶产品具体buffer活性表 热度: NEB酶切buffer 热度: 窗体顶端 Select1stenzyme: Select2ndenzyme: 窗体底端 Enzyme Cat# Temp SuppliedNEBuffer Supplements %ActivityinNEBuffer BSA SAM 1 2 3 4 BamHI* R0136(http:\/\/.neb\/...
一般来说，两个保护碱基就足够了。关于双酶切设计，我建议你使用double digestion designer，http://www...
NEB 2000年目录上双酶切表显示:BamH I 和 Neh I双酶切时用BamH I Buffer,而该网站(http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/double_digests.asp)上则显示要分开切。应该以哪个为准? 困惑2: BamH I 在Buffer2中活力为100%,Nhe I提供的就是Buffer2,为什么不能用Buffer2作为双酶...
NEB的酶一般没问题，切不开的常见原因：1 酶过多，导致甘油浓度过高，抑制酶活 2 保护碱基不足 3 buffer不对
Use this tool to select reaction conditions amenable to any two NEB restriction enzymes. C lick here for more information on double digests and a chart of double digests for some common enzyme combinations. Select 1st enzyme: Enzyme Cat# Temp Supplied NEBuffer Supplements % Activity in NE...
。酶的protocal上有写在NEB不同buffer中的酶活力，你选一个都是100%的就行了，NEB官网也有，http://www.neb.sg/tools-and-resources/interactive-tools/double-digest-finder，你这两种酶应该用BUFFER后期尝试了只用2.1但是效果也还是不行， 也换了孵育的时间。 跑胶的时候就还是 似乎是没有条带。