The US National Cancer Institute (NCI) 60 human tumour cell line anticancer drug screen (NCI60) was developed in the late 1980s as an in vitro drug-discovery tool intended to supplant the use of transplantable animal tumours in anticancer drug screening. This screening model was rapidly ...
Boyd MR: The NCI human tumor cell line (60 Cell)screen, in Teicher BA & Andrews PA: Anticancer Drug Development Guide Preclinical Screening, Clinical Trials, and Approval, 2nd ed. Humana Press, Totowa, NJ, 2004, pp 41–61.Boyd, M.R. The NCI Human Tumor Cell Line (60-Cell) Screen....
NCI-60是一组由美国国家癌症研究所使用的60个人类癌细胞系面板,用于体外筛选多达7,000种小分子(合成或纯化天然产物),以每年鉴定潜在的抗癌药物。这一药物筛选面板代表了人类癌症的多样化横截面,涵盖了白血病、黑色素瘤以及肺癌、结肠癌、脑癌、卵巢癌、乳腺癌、前列腺癌和肾癌等。NCI-60面板的开发与应用在癌症研究和...
The NCI60 screen was established in the 1980s and introduced in the 1990s as a screening service for the worldwide cancer research community. The screen features 60 human tumor cell lines that represent 9 cancer types including non-small cell lung, colon, central nervous system, ovarian, renal...
Over the past three decades, high-throughput phenotypic cancer cell line screens have revealed unanticipated small-molecule activities and illuminated connections between tumor genotypes and anticancer efficacy. Founded in 1984, the National Cancer Institute's "NCI60" screen laid the conceptual groundwork...
Herein, we present the methodology used to generate this extract library and the preliminary assessment of the biological activity in the NCI-60 Human Tumor Cell Line Screen. Chemical identification the active components of four most NCI-60 active TCM species was also conducted namely Brucea javanica...
This is not such a tumor-normal comparison study, but rather, one aimed to identify potential functions of mobile DNAs in human cancer cells. Towards this end, we mapped LINE-1 and Alu insertions in the NCI-60 tumor cell panel. We used a method for interspersed repeat mapping, Transposon...
Since 1990, the Developmental Therapeutics Program (DTP) of the National Cancer Institute (NCI) has screened over 60 000 compounds and a larger number of natural product extracts for their capacity to inhibit the growth of 60 different human tumor cell lines (1). These cell lines have been ma...
The in vitro anti-cancer drug cell line screen established at DTP is unique in several respects. It has changed the NCI emphasis from a compound-oriented drug discovery effort to a disease-panel oriented exercise, emphasized human tumor cells derived from solid tumors, developed a high volume ...
models to the integrated datasets of NCI-60 cell lines and discovered/reaffirmed prominent mechanistic links pertaining to major oncogenes and tumor suppressors including CDKN2A, TP53, MYB, MITF, PAX3, and other genes. Methods Overview of the integrated modeling framework...