Under a low-K+ diet, KO mice exhibit hypokalemia and volume depletion with increased blood CO2 levels and Renin mRNA levels. NCC expression and its phosphorylation are both increased in control and KO groups under low-K+ diet compared to standard diet but, the...
PCR was performed in a total reaction volume of 25 μL containing 100 ng of genomic DNA; 0.2 μM of each primer, 0.6 μL of Taq DNA polymerase, 0.5 μL of Taq DNA polymerase buffer, 2.5 mM Mg Cl2 (20 mM), and 0.2 μM dNTP. Table 1. 18S, 16S rDNA, rbcl, and 5.8S ...