To examine gene ablation efficiency of qgRNA and other reagents in various cell types and organoids, HCT116-Cas9 cells were grown in DMEM medium (GIBCO) supplemented with 10% FBS (GIBCO) and 1× penicillin/streptomycin (GIBCO) in a humidified incubator at 37 °C with 5% CO2. iPSC-iCas...
Cells were cultured in assay media and incubated for 1 h at 37 °C in a non-CO2 incubator. The Seahorse XF Mito Stress test was used to measure the oxygen consumption rate response after the sequential injection of oligomycin (1.0 μM), FCCP (1.0 μM) and Rot/AA (0.5...
At the same time, the right hind that was intra-articularly injected with hydrogels was analyzed independently, and the parameters indicating the right hind functions, including stand, print area, and mean intensity, were obtained. The aforementioned parameters showed no significant differences among ...
and distinguish timing of expression and role of Ltk signaling:aLtk Chromatoblast ModelbLtk Iridoblast Model. In these schema, potency of cells (number of fill colours) at different stages in PFR of the pigment cell lineages decreases down...
d, Model of the closed-state complex in front (left) and back (middle) cartoon view and in a top (N-terminal) surface view (right). e, Sketch comparing the DNA binding mode of the Rad24-RFC–9-1-1 (upper panel, this study) with that of the RFC–PCNA, based on the T4 clamp...
Working at 40× magnification, move the microscope stage so that the pipette is in the microinjection droplet (CZB·H) containing 10–15 oocytes (transferred from the batch of Step 6 in the CO2 incubator), optionally first washing it in a CZB·H droplet to remove PVP360 solution from the...
resolved by eSRRF processing (marked gray) is 360 nm in the lateral directions (x,y) and 500 nm in the axial direction (z). (iii) marks the displayed area of the temporal color-coded projection of a singlez-slice over the whole MFM (left) and MFM + eSRRF (right) acquisit...
temperature and was added drop-wise to the cells with gentle swirling. Sodium butyrate was then added at 8 mM final concentration. Cells were returned to the incubator and harvested 36 h post-transfection, expression for longer time periods (48 or 72 h) resulted in lower quality ...
Outlined regions are magnified on the right-hand side. Scale bar, 5 µm. c, Porcine oocytes expressing EGFP-Lamin B1 (not shown) and mScarlet-UtrCH (F-actin). Time series of the highlighted region is shown on the right-hand side. Coloured arrowheads point to actin cables in the ...
The plates were then automatically transferred to an integrated incubator and incubated for 5 days at 37 °C with 5% CO2, a time at which virus-induced cytopathic effect is 100% in the untreated, infected control conditions (virus controls). Uninfected assay ready compound plates were treated...