基于实验室已有基础,作者首先在乳酸乳球菌多药耐药性调节因子(LmrR)尝试插入含硼酸的非天然氨基酸。尽管该蛋白骨架缺乏天然催化功能,但由于其结构独特且可进化,已有多篇作为人工酶的骨架实现非天然反应的报道(Nature.2022,611, 715;Nat. Catal.2020,3, 289;Nat. Chem.2018,10, 946)。作者选择LmrR蛋白中的6个位...
Nature volume 611, pages 715–720 (2022)Cite this article 25k Accesses 114 Citations 51 Altmetric Metrics details Abstract Naturally evolved enzymes, despite their astonishingly large variety and functional diversity, operate predominantly through thermochemical activation. Integrating prominent photocatalysis ...
Nature 611, 709–714 (2022). Article ADS CAS PubMed MATH Google Scholar Sun, N. et al. Enantioselective [2+2]-cycloadditions with triplet photoenzymes. Nature 611, 715–720 (2022). Article ADS CAS PubMed MATH Google Scholar Emmanuel, M. A., Greenberg, N. R., Oblinsky, D. G....
1, 804–814 (2022). Article ADS Google Scholar Novikov, M. S., Khlebnikov, A. F., Krebs, A. & Kostikov, R. R. Unprecedented 1,3-dipolar cycloaddition of azomethine ylides derived from difluorocarbene and imines to carbonyl compounds − Synthesis of oxazolidine derivatives. Eur. J. ...
715 5,903 229,186 93,920 50,724 Journal Impact Factor 223.679 74.449 70.670 59.102 57.618 54.301 54.000 51.848 51.273 44.019 43.704 43.351 43.070 41.037 40.443 38.887 38.296 36.216 35.386 34.648 34.106 34.024 34.024 33.407 33.250 33.162 33.069 32.274 31.864 31.583 30.641 30.628 28.755 28.467 28.295 ...
A recent report suggested that in both homologous viral L230-LH domain and in human LH2, replacement of the fully conserved, surface-exposed C-terminal residue Leu715 (LH3 numbering, located in the middle of the “elongated” dimer interface found in the LH3 crystal structure) with a charged...
被誉为“中国天眼”的500米口径球面射电望远镜(FAST)正式验收至今已近两年。“中国天眼”运行效率和质量不断提高,年观测时长超过5300小时,已远超国际同行预期的工作效率,为其科学产出起到重要支撑作用。1月5日,中国科学院发布消息,2021年,科学家依托“中国天眼”又取得...
精确地计时无疑对生物的生存和繁殖至关重要。因此,大脑如何跟踪并记录时间的奥秘是神经科学研究领域一个重要的根本性问题,而我们对其的了解在很大程度上仍然知之甚少。特别是计时的行为学机制,以及介导计时的大脑环路和神经计算机制有待阐明。金鑫教授团队通过结合小鼠的行为分析、体内电生理学、遗传和光遗传学工具,揭示...
尽管该蛋白骨架缺乏天然催化功能,但由于其结构独特且可进化,已有多篇作为人工酶的骨架实现非天然反应的报道(Nature.2022,611, 715;Nat. Catal.2020,3, 289;Nat. Chem.2018,10, 946)。作者选择LmrR蛋白中的6个位置(8、11、15、89、92和93)用于pBoF的插入,使用羟胺作为简单亲核试剂,与适当的α-羟基酮可形成...
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