These reactions can selectively convert N-terminal Gly-containing oligopeptides and N-terminal Ile-containing oligopeptides to fluorescence (FL) compounds, respectively, but not proteins, acetyl peptides or amino acids. Under optimized conditions using 1.65 渭M collagen IV or 1.5 mM Ac-GPQGI- AGQ ...
B. (1993) Solid-phase synthesis and stability of triple-helical peptides incorporating native collagen sequences. Biopolymers 33 , 1695–1707.Fields CG, Lovdahl CM, Miles AJ, Matthias-Hagen VL, Fields GB (1993) Solid-phase synthesis and stability of triple-helical peptides incorporating native ...
The properties of the arterial wall depend on the mechanical role of each of its passive (elastin and collagen fibers) and active (SMCs) components. These components determine the vessel’s elastic, viscous, and inertial properties, though the inertial component is negligible [16]. Consequently, ...
Therefore, the less raised T1 value found in patients with ATTR amyloidosis could be due to (amongst others), a lower amyloid burden, less hydration of the amyloid, less collagen associated with amyloid, or differential effects on the intracellular signal. Finally, AL may have additional ...
Ivanisevic, Modification of native collagen with cell‐adhesive peptide to promote RPE cell attachment on Bruch's membrane, Biotechnol. Bioeng. 102 (2009) 1723-1729.R. Sistiabudi, J. Paderi, A. Panitch, A. Ivanisevic. Modification of native collagen with cell adhesive peptide to promote...
Here, a novel assay for human collagenase activity is described in which enzymatic degradation of native collagen or acetyl peptide is determined by using a fluorogenic reaction for oligopeptides. The oligopeptides are quantified spectrofluorometrically with either 3,4-dihydroxyphenylacetic acid or 1,2-...
Quantitative Liver Fibrosis Using Collagen Hybridizing Peptide to Predict Native Liver Survival in Biliary Atresia: A Pilot Study.doi:10.1097/mpg.0000000000002505Catalina JaramilloStephen L. GutheryAmy LowichikGregory StoddardTaegun KimYang LiMichael Kyle Jensen...
Video S1. Illustrations of 2pSAM with multi-site aberration correction compared to traditional TPM, related to Figures 1, 2, S1, and S2 and STAR Methods Video S2. Evaluation of photobleaching and phototoxicity between TPM and 2pSAM, related to Figures 4 and S4 Video S3. Comparison between TP...