46were prepared by dissolving RA(0.4 mg) or RB(1.0 mg) in a sodium bicarbonate buffer solution (25 mM at pH 10.0, 120 μL) containing urea (1 or 2 M, respectively) and EDTA (1 mM). The concentrations of RAand RBwere determined...
Following amplification, the PCR products were submitted to electrophoresis on a 1% agarose gel in TBE buffer 1X (Carl Roth) for 35 min under an electrical voltage of 135 mV and visualized under UV with the U: GENIUS3 gel reader (Syngene, Cambridge, UK). Successfully amplified PCR were ...
Eleven endophytic bacteria were isolated from the roots of cumin, a medicinal plant grown in Assiut Province, Egypt. The strains KAU2025 and KAU2022 exhibited the highest inhibition rate against Fusarium oxysporum (FOC7) (64%) in dual culture test and we