Reconstituted lipids were then mixed with 1×TBS and scaffold protein MSP1D1 (BioNanoCon, Lot #150221B or Sigma Catalog # M6574) and incubated at RT for 30 minutes. Disc reconstitution mixtures were prepared according to the recipe (all volumes in μl) shown in Table 4.TABLE 4 Disc rec...
The theoretical whey fraction is based on the casein/whey protein ratio of the product, from the recipe of the product. Example 3: Protein Profile Analysis The protein composition of both fresh and digested EH-WPC and MP-WPC was evaluated by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) ...
The PCR insert and plasmid pMIP_MP-Be_His (2) were both digested with XhoI and SpeI, gel-purified, and ligated to yield plasmid pMIP_Npu, which was sequenced to verify that the intein was fused in between MBP and paramyosin-Δ Sal. The cis-splicing precursor was then converted into a...
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