Protocol: NativePAGE Bis-Tris Gels Cookie设置 我们及我们的关联方等通过运用Cookie等技术来为您提供您感兴趣的网站定制内容、识别访问者、保障安全登录和收集数据。点击“全部接受”以接受所有 cookie 并直接跳转至网站,或点击“管理设置”以查看 cookie 类型的详细说明,并选择是否在网站上接受某些 cookie。
Protocol: NativePAGE Bis-Tris Gels NativePAGE Novex Bis-Tris Gel System 在此網站使用 Cookie 的選擇 我們以及我們的附屬公司和供應商使用 cookies 和類似技術來經營我們的網站、識別我們網站的訪客、提供安全登入、收集統計資料以優化網站功能,並提供根據您的興趣量身定制的內容。點擊「全部接受」 以接受所有 cooki...
Native chromatin immunoprecipitation protocol 实验概要 The method is a native chromatin immunoprecipitation protocol. 主要试剂 1. 10 x TBS 0.1 M Tris-HCl (pH 7.5) 1.5 M NaCl 30 mM CaCl2 20 mM MgCl2 50 mM Na butyrate (pH 8.0) 2. Digestion buffer 0.32 M sucrose ...
A new protocol for conducting two‐dimensional (2D) electrophoresis was developed by combining the recently developed agarose native gel electrophoresis with either vertical sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis (PAGE) or flat SDS agarose gel electrophoresis. Our innovative ...
Protocol Estimating target protein expression level If this is the first time you have prepared clarified samples from cells expressing a particular recombinant protein, we recommend that you estimate the protein's expression level in that host strain as follows before preparing ...
Phenotypic data were collected from the n = 381 sexually mature lizards in our AL population sample: hind limb length (HLL) and snout-to-vent length (SVL) were each measured to the nearest 0.5 mm using a ruler following a protocol described in ref. 17. DNA extraction All the ...
K. (1998). A quantitative cryogenic gel-shift technique for analysis of protein-DNA binding. Anal Biochem 258:240-245. PubMed CAS Google Scholar Barkley, M. (1981). Salt dependence of the kinetics of the lac repressor-operator interaction: role of nonoperator deoxyribonucleic acid in the ...
尺寸排阻色谱(SEC)与非变性电喷雾电离质谱(Native ESI-MS)联用技术是研究天然蛋白质的有效工具。但已报道的研究大多集中于技术应用,并未验证蛋白质在所用分离条件下是否处于原始状态,也未评估SEC洗脱条件对蛋白质-固定相的相互作用和蛋白质变性的影响。近期发表在Analytical Chemistry的一篇文章深入分析了挥发性流动相...
On the other hand, applying our optimized expansion microscopy protocol U-ExM10 on unfixed cells preserves the ultrastructure of the centriole, a stable microtubule-based organelle; however, we also observed that the complete lack of fixation in living cells leads to the loss of some cellular ele...
For a 5ml native PAGE stacking gel *: 在每次使用前添加。 For a 10ml native PAGE separating gel: *: 在每次使用前添加。 Sample buffer (2x): Running Buffer: 注意:运行缓冲液应为~ pH 8.3。不要调节 pH 值。 凝胶电泳方案 1. 在小烧杯中准备适量的分离胶,然后加入特定体积的分离胶。AP 和 TEMED...