Marker set of non-denaturing gel is provided. The protein molecular weight marker is provided in liquid form, it is stable for at least 1 year 2 months, at -20 ℃ at least in the liquid state of 4 ℃. Also, how to use the kit comprises a stable native protein marker liquid and ...
For a 10ml native PAGE separating gel: *: 在每次使用前添加。 Sample buffer (2x): Running Buffer: 注意:运行缓冲液应为~ pH 8.3。不要调节 pH 值。 凝胶电泳方案 1. 在小烧杯中准备适量的分离胶,然后加入特定体积的分离胶。AP 和 TEMED 并轻轻旋转烧杯以确保充分混合。将凝胶溶液移入凝胶浇注的玻璃板...
配。细胞超声即可,超声后离心取上清.也有配NATIVE-PAGE gel所用buffer为1*TBE,用的running buffer也是1*TBE。还有一点就是要在配gel时考虑能使蛋 白多聚体稳定的因素。 4. 做了naive---PAGE没有条带,蛋白质是纯品,分子量很大,怎么回事呢? 因为分子量很大,8%的胶浓度较合适。加样时加个marker,可以检测胶...
It is a ready-to-use protein marker that allows for molecular weight estimation of proteins using native gel electrophoresis. Please note that even with a native standard, molecular weight estimation in native electrophoresis is very inaccurate, as gel migration can be significantly affected by ...
DescriptionHMW Native Marker Kit Product TypeHMW Native Marker Kit For Use With (Application)For MW determinations by polyacrylamide gel electrophoresis (PAGE) in homogeneous or gradient gels. Molecular Weight (g/mol)Mr 669 000 Storage RequirementsUnopened vials should be stored at 4°C ...
HMW Native Marker(66-669) Suppliers list Company Name:BEIJING BIOSYNTHESIS BIOTECHNOLOGY CO., LTD. Tel:400-901-9800 Email:sales@bioss.com.cn Company Name:Shanghai Guyan Industrial Co., Ltd. Tel:021-39921927 18321818584 Email:3004901493@qq.com ...
产品属性 英文名NativeMark™ Unstained Protein Standard 数量2254 供应商明阳科华 产品说明 Description The NativeMark™ Unstained Protein Standard is a ready-to-use protein standard designed for molecular weight estimation of proteins using native gel electrophoresis. Features of this marker: ...
(B) 1 µg of each fraction was resolved on a 15% denaturing acrylamide gel and stained with ethidium bromide. After electrotransfer, miR-16 miRNA was detected by Northern blot with an antisense RNA probe 5' labeled and purified with the mirVana™ Probe & Marker Kit (Am...
DNA marker 常规溶液 其他试剂盒 猪ELISA试剂盒 细胞活力/毒性检测试剂盒 小鼠ELISA试剂盒 大鼠ELISA试剂盒 人ELISA试剂盒 蛋白提取相关试剂盒 核酸类 蛋白类 联系我们 联系人:常婕 点击查看联系方式 供应产品 Native Gel Sample Loading Buffer(2×) 1共1页 推荐产品 我要推广优质信息推广广告 有氧厌氧呼吸...
Cells expressing the ER marker GFP-Sec61β are stained with α/β-tubulin (cyan) and GFP (yellow). White dotted squares correspond to the adjacent insets. U-ExM, ultrastructure expansion microscopy. Scale bars, 5 μm and 2 μm (insets). Full size image Cryofixation surpasses ...