Furthermore, we also demonstrated that the FlipNanoLuc-based coronavirus 3CL-protease sensor can be used to detect human coronavirus OC43 with tenfold increase in luminescence and severe acute respiratory syndrome-coronavirus-2 infections with 20-fold increase in luminescence by introducing the stem-...
After 48 h, vector transduction was examined based on the expression of luciferase or secNanoLuc. The mean value of the triplicate samples was considered as one experiment. Inhibition of vector transduction by Nabs was expressed as the percentage of transduction; 100% meant no inhibition, whereas ...
This chapter describes two NanoLuc-based methods to monitor β-arrestin2 recruitment to the human histamine H1 receptor by measuring bioluminescence resonance energy transfer and enzyme-fragment complementation in real-time on living cells with reasonable high throughput. In addition to the detection of ...
NanoLuc luciferaseBioluminescenceUltrabrightUltrasensitivePbNLucBioluminescence imaging is widely used for cell-based assays and animal imaging studies, both in biomedical research and drug development. Its main advantages include its high-throughput applicability, affordability, high sensitivity, operational ...
The Use of a Novel NanoLuc -Based Reporter Phage for the Detection of Escherichia coli O157:H7. Sci Rep, 6, 33235.ZHANG D, CORONELAGUILERA C P, ROMERO P L, et al. The use of a novel nanoLuc-based reporter phage for the detection of Escherichia coli O157: H7[J]. Scientific Reports...
To address this issue, we developed a family of new brain-permeant NanoLuc (NLuc) substrates, cephalofurimazine (CFz). CFz family paired with Antares, a fusion of CyOFP and NLuc that emits orange light instead of blue, produces an order of magnitude more signal from the brain than the ...
This study aimed to develop a novel yeast-based assay using a codon-optimized stable or unstable NanoLuc luciferase (yNluc and yNluCP) gene linked to a DNA damage- or oxidative stress-responsive promoter, enabling convenient sensing genotoxicity or oxidative stress, respectivel...
A split NanoLuc complementation-based human norovirus-like particle entry assay facilitates evaluation of anti-norovirus antibodies in live cellsWeihua QiaoShuxia WangChao ZhangZhong Huang
Herein, we introduced a nitroaryl group on the C-3 position and a hydroxy group at the C-6 phenyl ring on furimazine to report the first bioluminescent probe (7) based on NanoLuc-furimazine bioluminescent pair for the detection of nitroreductase in bacteria. The probe, which po...
(in the range of tens of oocysts) is challenging using firefly luciferase, NanoLuc-based luminescence in PbNLuc parasites allowed detection of a clear progressive increase in intensity from days 7–9 post-feed, which decreased again at day 15 coinciding with sporozoite egress and migration to ...