Probing histone modifications at a single-cell level in thousands of cells has been enabled by technologies such as single-cell CUT&Tag. Here we describe nano-CUT&Tag (nano-CT), which allows simultaneous mapping of up to three epigenomic modaliti
reduced the CD105 expression in the tumor tissues, and increased tumor cells apoptosis.aTumors were collected from mice bearing Bel7404 subcutaneous xenografts treated with CD105 CAR, CD19 CAR, Mock, Utd, or PBS. Formalin-fixed, paraffin-embedded tumor sections were consecutively cut and stained ...
Probing histone modifications at a single-cell level in thousands of cells has been enabled by technologies such as single-cell CUT&Tag. Here we describe nano-CUT&Tag (nano-CT), which allows simultaneous mapping of up to three epigenomic modaliti
The cut‑off value of the cELISA is 20.72 %. Three hundreds and sixty porcine serum samples were tested by both newly developed cELISA and commercial kits. The sensitivity and specificity were 99.68 % and 95.92 %, respectively. The coincidence rate of the two methods was 99....
Aggregation and spreading of α-Synuclein (αSyn) are hallmarks of several neurodegenerative diseases, thus monitoring human αSyn (hαSyn) in animal models or cell cultures is vital for the field. However, the detection of native hαSyn in such systems
(i) mixing two T cell lines, each expressing one CAR specific for one antigen (mixing); (ii) transducing T cells to simultaneously express two different CARs (dual signaling CAR, combining) or (iii) transducing T cells to express one single CAR that consists of two antigen-binding domains ...