Moreover, we show that the nucleotide conductance spectrum is affected little by its orientation inside the graphene nanopore. The proposed technique may be extremely useful for real applications in developing ultrafast, low-cost DNA sequencing methods.Anton Simeonov...
Ping Li, Jialin Li, Liu Qin, Nano2NGS-Muta: a framework for converting nanopore sequencing data to NGS-liked sequencing data for hotspot mutation detection, NAR Genomics and Bioinformatics, Volume 4, Issue 2, June 2022, lqac033, https://doi.org/10.1093/nargab/lqac033...
Xenium技术的本质是基于FISSEQ(Fluorescent in situ Sequencing,荧光原位测序),其核心就是3步。 首先组织切片预处理后,进行探针杂交,洗掉未结合探针后进行滚环复制扩增(RCA,Rolling circle amplification)。 这一步用到的探针包含部分,一部分是两端与目标片段互补,用于结合目标片段,另外一部分包含已知序列,用来与后面的荧...
This option will carry all the pre-processing of sequencing data and the NanoSeq analysis. Both duplex and normal FASTQs are treated as NanoSeq libraries. Sample sheet format id,d_fastq1,d_fastq2,n_fastq1,n_fastq2 experiment1,1_duplex_R1.fastq.gz,1_duplex_R2.fastq.gz,1_normal_R1....
相比水凝胶墨水,PN film促进了真皮成纤维细胞的粘附和增殖。Bulk RNA sequencing的结果表明,PN film激活了真皮成纤维细胞的MAPK, Hippo, TGF-β, PI3K-Akt等组织修复相关通路,上调了细胞的粘附、增殖、迁移和基质分泌等组织修复相关基因表...
该研究在国际上率先开发了一种基于单分子长读段测序平台(a single-molecule sequencing platform,SMS)、在单细胞分辨率研究蛋白质-DNA相互作用的新方法,称为scNanoSeq-CUT&Tag。使用scNanoSeq-CUT&Tag技术,该研究对体外培养的六种有代表...
光学基因组图谱技术(Optical genome mapping, OGM)是一种新型高分辨率细胞遗传学分析技术,该技术可以利用单个DNA分子基因组限制性内切酶图谱快速生成高分辨率、有序的全基因组限制性内切酶图谱,在检测基因组结构变异的方面具有重大应用价值;这一技术进而可以与现有的高通量测序技术(Next generation sequencing, NGS)相结合,...
(1.88 ± 0.16 of Ost-EVs relative to Nor-EVs) and the formation of orange-red calcium nodules (1.38 ± 0.10 of Ost-EVs relative to Nor-EVs) in vitro. RNA sequencing revealed that Ost-EVs showed significantly high ...
et al. Fully phased human genome assembly without parental data using single-cell strand sequencing and long reads. Nat. Biotechnol. 39, 302–308 (2021). 3. Guarracino, A., Heumos, S., Nahnsen, S., Prins, P. & Garrison, E. ODGI: understanding pangenome graphs. Bioinformatics 38, ...
2.5 dollars per cell under current sequencing depth. As the cost of ONT is still higher than that of NGS platform at the same sequencing depth, there is a trade-off between more fragments to enrich stronger epigenetic signals and longer reads to detect long read-specific features such as SVs...